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MB Sample ID: SA320903
| Local Sample ID: | WT1 |
| Subject ID: | SU003060 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Species Group: | Mammals |
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Sample Preparation:
| Sampleprep ID: | SP003066 |
| Sampleprep Summary: | As described in the provided protocol (see attached file): - "2mL chloroform:methanol (2:1, v/v) was added to user provided tissue. The sample was then sonicated under ice chilled probe sonicator for 20 sec, cooled down for 10 sec and another sonication for 20 sec. The sample was then centrifuged at 3000 g for 5 min. Supernatant was aliquot out and dried under nitrogen.The sample was then reconstituted with IPA:MeOH:chloroform (1:1:0.2, v/v) in 1mg to 5uL. Then, 3 μL was injected into the LC-MS/MS system." |
| Sampleprep Protocol Filename: | untargeted_protocol.pdf |
