Summary of Study ST000135

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000120. The data can be accessed directly via it's Project DOI: 10.21228/M82P4X This work is supported by NIH grant, U2C- DK119886.

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Study IDST000135
Study TitleLCMS analysis of Bile Acids
Study Typedrug dosage
Study SummaryMice were dosed with TCDF for 5 days
Institute
Pennsylvania State University
DepartmentMolecular Toxicology
Last NamePatterson
First NameAndrew
Emailadp117@PSU.EDU, RGN5011@psu.edu
Submit Date2015-01-13
Num Groups5
Total Subjects58
Raw Data AvailableNo
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2015-01-13
Release Version1
Andrew Patterson Andrew Patterson
https://dx.doi.org/10.21228/M82P4X
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000120
Project DOI:doi: 10.21228/M82P4X
Project Title:Aryl Hydrocarbon Receptor Activation by Persistent Organic Pollutants Impacts Gut Microbiota-Host Metabolic Homeostasis in Mice
Institute:Pennsylvania State University
Department:Molecular Toxicology
Last Name:Patterson
First Name:Andrew
Email:adp117@PSU.EDU; RGN5011@psu.edu

Subject:

Subject ID:SU000154
Subject Type:Animal
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BL/6J
Age Or Age Range:6 weeks old
Gender:Males
Species Group:Mammal

Factors:

Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Gender Genotype
SA007399601 IleumMale Tcdf Treated
SA007400404 FecesMale Tcdf Treated
SA007401402 IleumMale Tcdf Treated
SA007402603 FecesMale Tcdf Treated
SA007403604 IleumMale Tcdf Treated
SA007404601 FecesMale Tcdf Treated
SA007405402 CecumMale Tcdf Treated
SA007406604 CecumMale Tcdf Treated
SA007407402 FecesMale Tcdf Treated
SA007408601 CecumMale Tcdf Treated
SA007409401 FecesMale Tcdf Treated
SA007410404 CecumMale Tcdf Treated
SA007411603 CecumMale Tcdf Treated
SA007412602 CecumMale Tcdf Treated
SA007413603 IleumMale Tcdf Treated
SA007414404 IleumMale Tcdf Treated
SA007415601 JejunumMale Tcdf Treated
SA007416404 LiverMale Tcdf Treated
SA007417602 JejunumMale Tcdf Treated
SA007418603 JejunumMale Tcdf Treated
SA007419604 JejunumMale Tcdf Treated
SA007420603 LiverMale Tcdf Treated
SA007421601 LiverMale Tcdf Treated
SA007422602 LiverMale Tcdf Treated
SA007423602 IleumMale Tcdf Treated
SA007424402 JejunumMale Tcdf Treated
SA007425404 JejunumMale Tcdf Treated
SA007426403 FecesMale Tcdf Treated
SA007427604 LiverMale Tcdf Treated
SA007428102 FecesMale Wild Type
SA007429302 JejunumMale Wild Type
SA007430303 JejunumMale Wild Type
SA007431304 JejunumMale Wild Type
SA007432103 FecesMale Wild Type
SA007433301 JejunumMale Wild Type
SA007434203 JejunumMale Wild Type
SA007435202 JejunumMale Wild Type
SA007436304 CecumMale Wild Type
SA007437304 IleumMale Wild Type
SA007438202 IleumMale Wild Type
SA007439203 IleumMale Wild Type
SA007440302 IleumMale Wild Type
SA007441301 IleumMale Wild Type
SA007442301 LiverMale Wild Type
SA007443303 LiverMale Wild Type
SA007444304 LiverMale Wild Type
SA007445302 LiverMale Wild Type
SA007446303 IleumMale Wild Type
SA007447301 CecumMale Wild Type
SA007448202 FecesMale Wild Type
SA007449203 FecesMale Wild Type
SA007450201 FecesMale Wild Type
SA007451202 CecumMale Wild Type
SA007452203 CecumMale Wild Type
SA007453302 CecumMale Wild Type
SA007454303 CecumMale Wild Type
SA007455201 LiverMale Wild Type
SA007456204 FecesMale Wild Type
Showing results 1 to 58 of 58

Collection:

Collection ID:CO000138
Collection Summary:-
Sample Type:Feces, liver, small and large intestine

Treatment:

Treatment ID:TR000156
Treatment Summary:Wild type and AHR knock out mice (5-6 per group) were treated with TCDF for 5 days
Treatment:Dietary
Treatment Compound:TCDF
Treatment Route:Oral
Treatment Dose:24 µg/kg body weight

Sample Preparation:

Sampleprep ID:SP000151
Sampleprep Summary:-
Sampleprep Protocol Filename:Bile_Acid_Extraction_for_LCMS.docx
Processing Method:Homogenization
Processing Storage Conditions:-20
Extraction Method:Ethanol extraction
Sample Spiking:Used D4 standards of Cholic Acid , Lithocholic Acid, Ursodeoxycholic Acid, Chenodeoxycholic Acid, and Deoxycholic Acid
Organ:Liver, small and large intestine, and feces

Combined analysis:

Analysis ID AN000217
Analysis type MS
Chromatography type
Chromatography system
Column
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Waters
Ion Mode POSITIVE
Units Peak area

Chromatography:

Chromatography ID:CH000149

MS:

MS ID:MS000169
Analysis ID:AN000217
Instrument Name:Waters
Instrument Type:Triple quadrupole
MS Type:ESI
Ion Mode:POSITIVE
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