Summary of Study ST000585

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000428. The data can be accessed directly via it's Project DOI: 10.21228/M8QW3H This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000585
Study TitleMetabolomic profiling of follicular fluid from patients with infertility-related deep endometriosis.
Study Summarythe metaboloc qualitative profiling was performed by LC-MS in follicular fluid samples of controls and endometriosis patients undergoing in vitro fertilization treatment
Institute
Sao Paulo Federal University
Last NameCordeiro
First NameFernanda
AddressRua Embau, 231, Vila Clementino
Emailfernandabertuccez85@gmail.com
Phone11996667402
Submit Date2017-04-06
Num Groups2
Total Subjects40
Num Females40
Raw Data AvailableYes
Raw Data File Type(s)raw(Waters)
Analysis Type DetailLC-MS
Release Date2017-07-10
Release Version1
Fernanda Cordeiro Fernanda Cordeiro
https://dx.doi.org/10.21228/M8QW3H
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR000428
Project DOI:doi: 10.21228/M8QW3H
Project Title:Follicular fluid Metabolomics
Project Type:MS qualitative analysis
Project Summary:Metabolomics profiling of follicular fluid samples from patients undergoing in vitro fertilization treatment
Institute:Sao Paulo Federal University
Department:Department of Surgery, Division of Urology, Human Reproduction Section
Laboratory:Center Of Research In Urology
Last Name:Cordeiro
First Name:Fernanda
Address:Rua Embau, 231, Vila Clementino
Email:fernandabertuccez85@gmail.com
Phone:11996667402

Subject:

Subject ID:SU000608
Subject Type:Human Follicular Fluid
Subject Species:Homo sapiens
Taxonomy ID:9606
Age Or Age Range:18-40
Gender:female
Human Medications:Controled ovarian Stimulation
Human Exclusion Criteria:Polycystic ovary syndrome, cancer, premature ovarian failure.
Species Group:Human

Factors:

Subject type: Human Follicular Fluid; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample Type
SA031694C14_2Control
SA031695C15_1Control
SA031696C16_1Control
SA031697C14_1Control
SA031698C15_2Control
SA031699C13_1Control
SA031700C11_2Control
SA031701C12_1Control
SA031702C12_2Control
SA031703C16_2Control
SA031704C13_2Control
SA031705C17_2Control
SA031706C20_2Control
SA031707C21_2Control
SA031708C22_1Control
SA031709C22_2Control
SA031710C20_1Control
SA031711C19_2Control
SA031712C11_1Control
SA031713C18_1Control
SA031714C18_2Control
SA031715C19_1Control
SA031716C17_1Control
SA031717C21_1Control
SA031718C04_1Control
SA031719C04_2Control
SA031720C05_1Control
SA031721C05_2Control
SA031722C03_2Control
SA031723C03_1Control
SA031724C01_1Control
SA031725C01_2Control
SA031726C02_2Control
SA031727C06_1Control
SA031728C02_1Control
SA031729C09_1Control
SA031730C09_2Control
SA031731C10_1Control
SA031732C10_2Control
SA031733C08_1Control
SA031734C08_2Control
SA031735C06_2Control
SA031736C07_1Control
SA031737C07_2Control
SA031738END13_1Endometriosis patient
SA031739END11_2Endometriosis patient
SA031740END12_2Endometriosis patient
SA031741END11_1Endometriosis patient
SA031742END12_1Endometriosis patient
SA031743END09_1Endometriosis patient
SA031744END08_2Endometriosis patient
SA031745END13_2Endometriosis patient
SA031746END09_2Endometriosis patient
SA031747END10_1Endometriosis patient
SA031748END10_2Endometriosis patient
SA031749END16_1Endometriosis patient
SA031750END17_1Endometriosis patient
SA031751END17_2Endometriosis patient
SA031752END08_1Endometriosis patient
SA031753END18_1Endometriosis patient
SA031754END16_2Endometriosis patient
SA031755END18_2Endometriosis patient
SA031756END14_2Endometriosis patient
SA031757END15_1Endometriosis patient
SA031758END15_2Endometriosis patient
SA031759END14_1Endometriosis patient
SA031760END03_2Endometriosis patient
SA031761END03_1Endometriosis patient
SA031762END04_1Endometriosis patient
SA031763END07_2Endometriosis patient
SA031764END02_1Endometriosis patient
SA031765END01_1Endometriosis patient
SA031766END01_2Endometriosis patient
SA031767END04_2Endometriosis patient
SA031768END02_2Endometriosis patient
SA031769END07_1Endometriosis patient
SA031770END05_1Endometriosis patient
SA031771END06_2Endometriosis patient
SA031772END06_1Endometriosis patient
SA031773END05_2Endometriosis patient
SA031774QC_Control_1Quality control
SA031775QC_Control_3Quality control
SA031776QC_Control_2Quality control
SA031777QC_Control_6Quality control
SA031778QC_END_1Quality control
SA031779QC_Control_4Quality control
SA031780QC_END_3Quality control
SA031781QC_END_5Quality control
SA031782QC_Control_5Quality control
SA031783QC_END_4Quality control
SA031784QC_END_6Quality control
SA031785QC_END_2Quality control
Showing results 1 to 92 of 92

Collection:

Collection ID:CO000602
Collection Summary:The FF samples were collected based on follicular aspiration after ovarian controlled stimulation protocol, defined by the doctor prior to the begining of the treatment. Follicular fluid samples were collectd and centrifuged at 800 × g for 10 minutes to separate the fluid from follicular cells and the samples were maintained frozen in -20 °C until the metabolites extraction.
Sample Type:Follicular fluid

Treatment:

Treatment ID:TR000622
Treatment Summary:Does not apply. Patients were treated for infertility according to their medical needs. The present study has no interference on patients management and it is defined as a prospective case-control study.

Sample Preparation:

Sampleprep ID:SP000615
Sampleprep Summary:The FF samples were centrifuged at 800 × g for 10 minutes to separate the fluid from follicular cells and the samples were maintained frozen in -20 °C until the metabolites extraction. The Bligh & Dyer protocol (1959) was applied for proteins removal and was performed as herein: 50 µL of sample was placed in a microtube, followed by addition of 50 µL of milli-Q water, 125 µL of chloroform (CHCl3 - Merck Millipore, MA, EUA) and 250 µL of methanol (MeOH - Merck Millipore - Massachusetts, EUA). The mixture was vortexed for 30 seconds. Next, polar and apolar phases were separated by the addition of 100 µL of milli-Q water and 125 µL of CHCl3 (Merck Millipore, MA EUA). Both polar and apolar phases were collected and transferred to a new microtube. The final content was dried by using a speedvac (CentriVap Cold Trap, Labconco, MO, USA). Prior to the LC-MS analysis, the metabolites were recovered by using 100 µL of acetonitrile and water (ACN:H2O 1:1, v:v - Merk Millipore, MA, USA) and filtered in 0.22 μm PVDF filters (Merck Millipore, MA, EUA).

Combined analysis:

Analysis ID AN000900
Analysis type MS
Chromatography type Reversed phase
Chromatography system Ultima Q-TOF
Column Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
MS Type ESI
MS instrument type QTOF
MS instrument name Waters Ultima QTOF
Ion Mode POSITIVE
Units unspecified

Chromatography:

Chromatography ID:CH000640
Instrument Name:Ultima Q-TOF
Column Name:Waters Acquity HSS T3 (100 x 2.1mm,1.8um)
Column Temperature:35 °C
Solvent A:100% water; 0.1% formic acid
Solvent B:100% acetonitrile; 0.1% formic acid
Analytical Time:17 minutes
Capillary Voltage:3 kV
Chromatography Type:Reversed phase

MS:

MS ID:MS000802
Analysis ID:AN000900
Instrument Name:Waters Ultima QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:POSITIVE
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