Summary of Study ST000669
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000472. The data can be accessed directly via it's Project DOI: 10.21228/M82028 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000669 |
| Study Title | Characterization of Retinal Exudates in Coats Disease - lipid profile |
| Study Type | MS analysis |
| Study Summary | The aim of our study is to characterize the retinal lipid exudates through lipidomic assays. Lipidomic analysis will quantify and identify the retinal lipid exudates and provide a `lipid profile? of the exudates. The characterization of the retinal lipids will help in further understanding the pathogenesis of Coat?s disease. It may allow us to identify and therapeutically target a metabolic pathway to prevent retinal exudation abnormal in Coat?s disease. |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@med.umich.edu | |
| Phone | (734) 232-8175 |
| Submit Date | 2017-06-05 |
| Num Groups | 11 |
| Total Subjects | 4 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2017-12-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000472 |
| Project DOI: | doi: 10.21228/M82028 |
| Project Title: | Characterization of Retinal Exudates in Coats Disease |
| Project Type: | MS analysis |
| Project Summary: | The aim of our study is to characterize the retinal lipid exudates through lipidomic assays |
| Institute: | University of Michigan |
| Department: | Ophthalmology and Visual Sciences |
| Laboratory: | Besirli Lab |
| Last Name: | Besirli |
| First Name: | Cagri |
| Address: | Ann Arbor, MI |
| Email: | cbesirli@umich.edu |
| Phone: | 734-615-6099 |
Subject:
| Subject ID: | SU000692 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
Factors:
Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)
| mb_sample_id | local_sample_id | Disease | Matrix | Sample type |
|---|---|---|---|---|
| SA038496 | S00019676 | AS-Control-P | Plasma | Plasma |
| SA038497 | S00019675 | AS-Control-S | SRF | Fluids and Secretions |
| SA038498 | S00019672 | Coats | CB | Fluids and Secretions |
| SA038499 | S00019673 | Coats | CB | Fluids and Secretions |
| SA038500 | S00019674 | JB-Coats-P | Plasma | Plasma |
| Showing results 1 to 5 of 5 |
Collection:
| Collection ID: | CO000686 |
| Collection Summary: | - |
| Sample Type: | Retina |
Treatment:
| Treatment ID: | TR000706 |
| Treatment Summary: | - |
Sample Preparation:
| Sampleprep ID: | SP000699 |
| Sampleprep Summary: | - |
| Sampleprep Protocol Filename: | A004_-_Shotgun_Lipidomics.pdf |
Combined analysis:
| Analysis ID | AN001024 | AN001025 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Shimadzu CTO-20A Nexera X2 | Shimadzu CTO-20A Nexera X2 |
| Column | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple TOF | Triple TOF |
| MS instrument name | ABI Sciex 5600+ TripleTOF | ABI Sciex 5600+ TripleTOF |
| Ion Mode | NEGATIVE | POSITIVE |
| Units | peak area | peak area |
Chromatography:
| Chromatography ID: | CH000732 |
| Instrument Name: | Shimadzu CTO-20A Nexera X2 |
| Column Name: | Waters Acquity HSS T3 (50 x 2.1mm,1.8um) |
| Column Temperature: | 55 |
| Flow Rate: | 0.400uL/min |
| Solvent A: | 40% acetonitrile/60% water; 10 mM ammonium acetate |
| Solvent B: | 10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
MS:
| MS ID: | MS000919 |
| Analysis ID: | AN001024 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | NEGATIVE |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
| MS ID: | MS000920 |
| Analysis ID: | AN001025 |
| Instrument Name: | ABI Sciex 5600+ TripleTOF |
| Instrument Type: | Triple TOF |
| MS Type: | ESI |
| Ion Mode: | POSITIVE |
| Analysis Protocol File: | A004_-_Shotgun_Lipidomics.pdf |
