Summary of study ST000736

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000533. The data can be accessed directly via it's Project DOI: 10.21228/M8S10C This work is supported by NIH grant, U2C- DK119886.

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Study IDST000736
Study TitleMetabolic change in macrophages after efferocytosis
Study TypeMS analysis
Study SummaryStudy metabolic pathway alteration in macrophages after efferocytosis (when macrophages eating apoptotic cells). Peritoneal macrophages were purified and plated in the cell-culture dishes. After culturing overnight. Apoptotic cells were overlayed to macrophages for 2 hours, then un-engulfed cells were removed through washing. Macrophages were left in the plate for another 4 hours before being lifted from the plate.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups5
Total Subjects1
Raw Data AvailableYes
Raw Data File Type(s).d
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8S10C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Project:

Project ID:PR000533
Project DOI:doi: 10.21228/M8S10C
Project Title:Metabolic change in macrophages after efferocytosis
Project Type:MS analysis
Project Summary:To study metabolic change in macrophages after efferocytosis
Institute:Northwestern University
Department:Pathology
Laboratory:Thorp Lab
Last Name:Thorp
First Name:Edward
Address:Evanston, IL
Email:ebthorp@northwestern.edu
Phone:312-503-4309

Subject:

Subject ID:SU000759
Subject Type:Mouse
Subject Species:Mus musculus
Taxonomy ID:10090
Species Group:Mammal

Factors:

Subject type: Mouse; Subject species: Mus musculus (Factor headings shown in green)

mb_sample_id local_sample_id Treatment
SA041101S00020917apoptotic cells for 2 hours
SA041102S00020916apoptotic cells for 2 hours
SA041103S00020915apoptotic cells for 2 hours
SA041106S00020920ko treated with apoptotic cells 2hrs
SA041104S00020921ko treated with apoptotic cells 2 hrs
SA041105S00020922ko treated with apoptotic cells 2 hrs
SA041107S00020918ko untreated
SA041108S00020919ko untreated
SA041109S00020914none
SA041110S00020913none
Showing results 1 to 10 of 10

Collection:

Collection ID:CO000753
Collection Summary:-
Sample Type:Macrophages

Treatment:

Treatment ID:TR000773
Treatment Summary:-

Sample Preparation:

Sampleprep ID:SP000766
Sampleprep Summary:-
Sampleprep Protocol Filename:A011_-_Glycolysis-TCA-nucleotides.pdf

Combined analysis:

Analysis ID AN001150
Analysis type MS
Chromatography type HILIC
Chromatography system Agilent
Column Phenomenex Luna NH2 (150 x 1mm, 3um)
MS Type ESI
MS instrument type QTOF
MS instrument name Agilent 6520B QTOF
Ion Mode NEGATIVE
Units counts

Chromatography:

Chromatography ID:CH000810
Instrument Name:Agilent
Column Name:Phenomenex Luna NH2 (150 x 1mm, 3um)
Chromatography Type:HILIC

MS:

MS ID:MS001045
Analysis ID:AN001150
Instrument Name:Agilent 6520B QTOF
Instrument Type:QTOF
MS Type:ESI
Ion Mode:NEGATIVE
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