Summary of Study ST001139

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000703. The data can be accessed directly via it's Project DOI: 10.21228/M8T10P This work is supported by NIH grant, U2C- DK119886.


Show all samples  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST001139
Study TitleP4HA1 knockdown in the breast cell line MDA231 Gln metabolism (part VI)
Study Typeisotope tracer
Study SummaryDetermine the effect on glutamine metabolism of knocking down P4HA1 in the MDA231 cell line.
University of Kentucky
DepartmentMarkey Cancer Center
Last NameXiong
First NameGaofeng
AddressBBSRC361, 741 South Limestone, Lexington, KY 40536, USA
Submit Date2019-02-15
Study CommentsFor additional comments about the study, like listing a publication.
Raw Data AvailableYes
Raw Data File Type(s)fid
Analysis Type DetailNMR
Release Date2019-03-06
Release Version1
Gaofeng Xiong Gaofeng Xiong application/zip

Select appropriate tab below to view additional metadata details:


Project ID:PR000703
Project DOI:doi: 10.21228/M8T10P
Project Title:P4HA1 knockdown in the breast cell line MDA231
Project Summary:Determine the effect of knocking down P4HA1 in the MDA231 cell line (including alterations to metabolic pathways).
Institute:University of Kentucky
Department:Markey Cancer Center
Last Name:Xu
First Name:Ren
Address:BBSRC361, 741 South Limestone, Lexington, KY 40536, USA


Subject ID:SU001203
Subject Type:Animal
Subject Species:Homo sapiens
Taxonomy ID:9606


Subject type: Animal; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id
Showing results 1 to 6 of 6


Collection ID:CO001197
Collection Summary:Collection and quench of cells in culture
Collection Protocol ID:cell_quench_collection
Collection Protocol Filename:3A_Cells_Quench_Cell_Fan_20170412.pdf
Sample Type:cultured cells


Treatment ID:TR001218
Treatment Summary:Cells grown in unlabeled glutamine and infected with empty vector. Cells grown in unlabeled glutamine and infected with shP4HA1 lentivirus. Cells grown in labeled glutamine and infected with empty vector. Cells grown in labeled glutamine and infected with shP4HA1 lentivirus.
Treatment Protocol ID:12C14NGln_shCTL 12C14NGln_shP4HA1 13C15NGln_shCTL 13C15NGln_shP4HA1

Sample Preparation:

Sampleprep ID:SP001211
Sampleprep Summary:Polar extraction from cells. Protein extraction and quantification.
Sampleprep Protocol ID:polar_extraction protein_extraction
Sampleprep Protocol Filename:4B_Extract_Polar_Lipid_Prot_Fan_070417.pdf


Analysis ID:AN001869
Analysis Type:NMR
Num Factors:2
Num Metabolites:22
Units:normalized peak area


NMR ID:NM000147
Analysis ID:AN001869
Instrument Name:Agilent 600
Instrument Type:FT-NMR
NMR Experiment Type:HC-HSQC
Standard Concentration:0.5 mM
Spectrometer Frequency:600 MHz
NMR Probe:cryogenic triple resonance HCN
NMR Solvent:D20
NMR Tube Size:1.7 mm
Shimming Method:gradient shimming
Pulse Sequence:gHSQCAD
Water Suppression:presaturation
Chemical Shift Ref Cpd:DSS
Temperature:15 celsius
Acquisition Time:2 s
Relaxation Delay:4 s
Baseline Correction Method:Bernstein Polynomial