Summary of Study ST003553

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002188. The data can be accessed directly via it's Project DOI: 10.21228/M8SV5J This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST003553
Study TitleInvestigation of age-dependent changes in the gut of honeybee workers using targeted metabolomics for amino acid and biogenic amine determination
Study SummaryIn the honeybee guts we measured the concentrations of 20 amino acids and 6 biogenic amines. Tryptophan and its downstream metabolite kynurenine followed the same trends across the worker development, with a significant decrease in their concentrations from day 3 onwards. Serotonin, on the other hand, was significantly increased from day 1. Tyramine and octopamine showed significant increases in concentrations (octopamine for all age groups apart from Day 1; tyramine for all age groups), while their precursor tyrosine showed significantly decreased concentrations for all age groups except experienced foragers.
Institute
Helmholtz Centre for Environmental Research
DepartmentMolecular Systems Biology
Last NameEngelmann
First NameBeatrice
AddressPermoserstraße 15, Leipzipg, Saxony, 03418, Germany
Emailbeatrice.engelmann@ufz.de
Phone004934160251099
Submit Date2024-10-28
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2024-11-27
Release Version1
Beatrice Engelmann Beatrice Engelmann
https://dx.doi.org/10.21228/M8SV5J
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002188
Project DOI:doi: 10.21228/M8SV5J
Project Title:Comprehension of the age-dependent gut and brain interaction of honeybee workers by integration of multi omics approaches
Project Summary:In honeybees, division of labour is a key feature, with age-related behavioural transitions being closely associated with molecular changes in the brain, gut, and microbiota. In this study, to investigate these molecular changes and thus better understand their contribution to behavioural responses and modulation, we analysed the global metabolomic shifts in honeybee workers and their microbiota throughout their lives. Overall, our findings provide new insights toward developing potential biomarkers for evaluation of different functional changes related to various environmental stressors.
Institute:Helmholtz Centre for Environmental Research
Department:Molecular Systems Biology
Last Name:Engelmann
First Name:Beatrice
Address:Permoserstraße 15, Leipzipg, Saxony, 03418, Germany
Email:beatrice.engelmann@ufz.de
Phone:004934160251099

Subject:

Subject ID:SU003682
Subject Type:Insect
Subject Species:Apis mellifera
Taxonomy ID:7460
Species Group:Insects

Factors:

Subject type: Insect; Subject species: Apis mellifera (Factor headings shown in green)

mb_sample_id local_sample_id life stage age of workers in days Sample source
SA388113H2_D1_3_dilutedestablishment 1 gut
SA388114H1_D1_3_dilutedestablishment 1 gut
SA388115H1_D1_4_dilutedestablishment 1 gut
SA388116H1_D1_5_dilutedestablishment 1 gut
SA388117H2_D1_1establishment 1 gut
SA388118H2_D1_2establishment 1 gut
SA388119H2_D1_3establishment 1 gut
SA388120H2_D1_4establishment 1 gut
SA388121H2_D1_5establishment 1 gut
SA388122H2_D1_1_dilutedestablishment 1 gut
SA388123H2_D1_2_dilutedestablishment 1 gut
SA388124H2_D1_5_dilutedestablishment 1 gut
SA388125H1_D1_1_dilutedestablishment 1 gut
SA388126H3_D1_1establishment 1 gut
SA388127H3_D1_2establishment 1 gut
SA388128H3_D1_3establishment 1 gut
SA388129H3_D1_4establishment 1 gut
SA388130H3_D1_5establishment 1 gut
SA388131H3_D1_1_dilutedestablishment 1 gut
SA388132H3_D1_2_dilutedestablishment 1 gut
SA388133H3_D1_3_dilutedestablishment 1 gut
SA388134H3_D1_4_dilutedestablishment 1 gut
SA388135H3_D1_5_dilutedestablishment 1 gut
SA388136H1_D1_2_dilutedestablishment 1 gut
SA388137H2_D1_4_dilutedestablishment 1 gut
SA388138H1_D1_5establishment 1 gut
SA388139H1_D1_4establishment 1 gut
SA388140H1_D1_3establishment 1 gut
SA388141H1_D1_1establishment 1 gut
SA388142H1_D1_2establishment 1 gut
SA388143H2_D3_3establishment 3 gut
SA388144H2_D3_2establishment 3 gut
SA388145H2_D3_1establishment 3 gut
SA388146H2_D3_3_dilutedestablishment 3 gut
SA388147H3_D3_1_dilutedestablishment 3 gut
SA388148H2_D3_4establishment 3 gut
SA388149H2_D3_5establishment 3 gut
SA388150H3_D3_2_dilutedestablishment 3 gut
SA388151H3_D3_3_dilutedestablishment 3 gut
SA388152H3_D3_4_dilutedestablishment 3 gut
SA388153H3_D3_5_dilutedestablishment 3 gut
SA388154H1_D3_2establishment 3 gut
SA388155H1_D3_1establishment 3 gut
SA388156H2_D3_2_dilutedestablishment 3 gut
SA388157H2_D3_5_dilutedestablishment 3 gut
SA388158H1_D3_2_dilutedestablishment 3 gut
SA388159H2_D3_1_dilutedestablishment 3 gut
SA388160H1_D3_3establishment 3 gut
SA388161H2_D3_4_dilutedestablishment 3 gut
SA388162H3_D3_5establishment 3 gut
SA388163H3_D3_4establishment 3 gut
SA388164H3_D3_3establishment 3 gut
SA388165H3_D3_2establishment 3 gut
SA388166H3_D3_1establishment 3 gut
SA388167H1_D3_1_dilutedestablishment 3 gut
SA388168H1_D3_3_dilutedestablishment 3 gut
SA388169H1_D3_4_dilutedestablishment 3 gut
SA388170H1_D3_5_dilutedestablishment 3 gut
SA388171H1_D3_4establishment 3 gut
SA388172H1_D3_5establishment 3 gut
SA388173H2_D5_1_dilutedestablishment 5 gut
SA388174H2_D5_5_dilutedestablishment 5 gut
SA388175H3_D5_5establishment 5 gut
SA388176H2_D5_4_dilutedestablishment 5 gut
SA388177H2_D5_3_dilutedestablishment 5 gut
SA388178H2_D5_2_dilutedestablishment 5 gut
SA388179H2_D5_4establishment 5 gut
SA388180H1_D5_1establishment 5 gut
SA388181H2_D5_5establishment 5 gut
SA388182H3_D5_4establishment 5 gut
SA388183H2_D5_3establishment 5 gut
SA388184H3_D5_1_dilutedestablishment 5 gut
SA388185H3_D5_3establishment 5 gut
SA388186H3_D5_2establishment 5 gut
SA388187H3_D5_1establishment 5 gut
SA388188H1_D5_1_dilutedestablishment 5 gut
SA388189H1_D5_2_dilutedestablishment 5 gut
SA388190H1_D5_3_dilutedestablishment 5 gut
SA388191H1_D5_4_dilutedestablishment 5 gut
SA388192H1_D5_5_dilutedestablishment 5 gut
SA388193H3_D5_5_dilutedestablishment 5 gut
SA388194H3_D5_4_dilutedestablishment 5 gut
SA388195H3_D5_3_dilutedestablishment 5 gut
SA388196H3_D5_2_dilutedestablishment 5 gut
SA388197H1_D5_2establishment 5 gut
SA388198H2_D5_2establishment 5 gut
SA388199H1_D5_5establishment 5 gut
SA388200H1_D5_4establishment 5 gut
SA388201H1_D5_3establishment 5 gut
SA388202H2_D5_1establishment 5 gut
SA388203H3_EF_4experienced_forager >12 gut
SA388204H3_EF_3experienced_forager >12 gut
SA388205H3_EF_2experienced_forager >12 gut
SA388206H3_EF_1experienced_forager >12 gut
SA388207H2_EF_1_dilutedexperienced_forager >12 gut
SA388208H2_EF_2_dilutedexperienced_forager >12 gut
SA388209H2_EF_3_dilutedexperienced_forager >12 gut
SA388210H2_EF_4_dilutedexperienced_forager >12 gut
SA388211H2_EF_5_dilutedexperienced_forager >12 gut
SA388212H3_EF_5experienced_forager >12 gut
Showing page 1 of 2     Results:    1  2  Next     Showing results 1 to 100 of 194

Collection:

Collection ID:CO003675
Collection Summary:Workers were pooled to obtain a final sample number of n = 5. The brain(s) and gut(s) from the same workers were assigned the same sample names. In total, we had 15 samples from each age group, with the exception of the foraging stages, where owing to the lack of foragers from Hive 1, we only had 11. All sampled bees were placed directly in liquid nitrogen to prevent changes in the metabolic profile. They were then decapitated in the laboratory so that the head and body of each bee were kept in a separate Eppendorf tube (Eppendorf, Germany) for later dissection. Worker guts were dissected on Petri dishes. Sternite 6 and the stinger apparatus were gently pulled using tweezers, which allowed extraction of the entire gastrointestinal tract. The stinger apparatus and venom sac were carefully excised and the honey crop was removed.
Sample Type:Bee gut

Treatment:

Treatment ID:TR003691
Treatment Summary:On the day of newly emerged workers (day 0), bees were samples as a baseline for no or low bacterial abundance in the gut. Moreover, bees from each hive were collected on Day 1, 3 and 5 to cover the establishment phase of the gut. Samples for the in-hive worker phase, defined as workers inside the hives that have a fully developed microbiome, were taken every day from Day 7 to Day 10. For the foraging phase, we differentiated between new and experienced foragers, as these show different behaviour due to different levels of experience.Based on experience, they were either collected one to two days after onset (new foragers) or at least four days after onset (experienced foragers).

Sample Preparation:

Sampleprep ID:SP003689
Sampleprep Summary:In brief, x mg pool gut was mixed with five times the volume (in µL) of acetonitrile (ACN):Water (1:1, v/v) and homogenized using a TissueLyser II (30 Hz, 10 min; Retsch Qiagen). After Centrifugation (2 min, 14000 rpm), 10 µL were used for amino acid derivatization. First, the supernatant was evaporated to dryness (SpeedVac, Eppendorf), resuspended in 50 µL of 5% phenyl isothiocyanate (PITC) in ethanol: H2O: pyridine (1:1:1, v/v/v), and incubated for 25 min at RT. Subsequently, the samples were dried to remove excess PITC and resuspended in 10 µL 5 mM ammonium acetate in methanol. After incubation (10 min at 14,000 rpm) 90 µL of H2O: ACN + 0.2% formic acid were added. Each derivative was measured in an undiluted and diluted (1:25) manner.

Combined analysis:

Analysis ID AN005840 AN005841
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Waters Acquity Waters Acquity
Column Zorbax Eclipse XDB-C18 (100 x 3.0mm, 3.5um) Zorbax Eclipse XDB-C18 (100 x 3.0mm, 3.5um)
MS Type ESI ESI
MS instrument type Triple quadrupole Triple quadrupole
MS instrument name ABI Sciex 5500 QTrap ABI Sciex 6500+ Qtrap
Ion Mode POSITIVE POSITIVE
Units µMol µMol

Chromatography:

Chromatography ID:CH004436
Instrument Name:Waters Acquity
Column Name:Zorbax Eclipse XDB-C18 (100 x 3.0mm, 3.5um)
Column Temperature:50
Flow Gradient:0-0.5 min at 0% B, 0.5-4 min 0-70% B, 4-5.3 min 70% B, 5.3-5.4 min 70-0% B, 5.4-7.3 min 0% B
Flow Rate:0.5 ml/min
Solvent A:100% water; 0.2% formic acid
Solvent B:100% acetonitrile; 0.2% formic acid
Chromatography Type:Reversed phase

MS:

MS ID:MS005560
Analysis ID:AN005840
Instrument Name:ABI Sciex 5500 QTrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:For identification and quantitation, a scheduled MRM method was used, with specific transitions for every amino acid and biogenic amine. Data acquisition and peak integration were performed in SciexOS software (Version 3.0.0.). Calculation of concentration was done using external calibration curves.
Ion Mode:POSITIVE
  
MS ID:MS005561
Analysis ID:AN005841
Instrument Name:ABI Sciex 6500+ Qtrap
Instrument Type:Triple quadrupole
MS Type:ESI
MS Comments:For identification and quantitation, a scheduled MRM method was used, with specific transitions for every amino acid and biogenic amine. Data acquisition and peak integration were performed in SciexOS software (Version 3.0.0.). Calculation of concentration was done using external calibration curves.
Ion Mode:POSITIVE
  logo