Summary of Study ST000272

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000216. The data can be accessed directly via it's Project DOI: 10.21228/M8GC7P This work is supported by NIH grant, U2C- DK119886.

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Study IDST000272
Study TitleAcyl-carnitine analysis (plasma)
Study TypeAdipose Acyl-carnitines
Study SummaryTo understand the contribution of adipose tissue fatty acid oxidation to whole-body metabolism, we generated mice with an adipose-specific knockout of carnitine palmitoyltransferase 2 (CPT2A?/?), an obligate step in mitochondrial long-chain fatty acid oxidation. CPT2A?/? mice became hypothermic after an acute cold challenge, and CPT2A?/? brown adipose tissue (BAT) failed to upregulate thermogenic genes in response to agonist-induced stimulation. The adipose-specific loss of CPT2 resulted in diet-dependent changes in adiposity but did not result in changes in body weight on low- or high-fat diets. Additionally, CPT2A?/? mice had suppressed high-fat diet-induced oxidative stress and inflammation in visceral white adipose tissue (WAT); however, high-fat diet-induced glucose intolerance was not improved. These data show that fatty acid oxidation is required for cold-induced thermogenesis in BAT and high-fat diet-induced oxidative stress and inflammation in WAT. http://www.sciencedirect.com/science/article/pii/S2211124714010523
Institute
University of Michigan
DepartmentBiological Chemistry(Johns Hopkins University School of Medicine)
LaboratoryWolfgang Lab(Johns Hopkins University School of Medicine)
Last NameWolfgang
First NameMichael
Address733 North Broadway, Suite G49 Baltimore, MD 21205-2196
Emailmwolfga1@jhmi.edu
Phone443-287-7680
Submit Date2014-02-21
Num Groups2
Total Subjects16
Study Comments(CPT2A?/?) stands for Carnitine palmitoyltransferase 2 knock out
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-01-06
Release Version1
Michael Wolfgang Michael Wolfgang
https://dx.doi.org/10.21228/M8GC7P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN000435
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1200
Column Waters Acquity HSS T3 (50 x 2.1mm, 1.8um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490 QQQ
Ion Mode POSITIVE
Units pmol/mg sample
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