Summary of Study ST000121
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000108. The data can be accessed directly via it's Project DOI: 10.21228/M8GW2Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000121 |
| Study Title | Impact of anesthesia and euthanasia on metabolomics of mammalian tissues: studies in a C57BL/6J mouse model |
| Study Type | Anesthesia effect study |
| Study Summary | We examined the effect of several commonly-used methods of anesthesia and euthanasia for collection of skeletal muscle, liver, heart, adipose and serum of C57BL/6J mice. The data revealed tissue-specific impacts of different anesthesia and euthanasia strategies. Based on these findings, we present a more optimal collection strategy mammalian tissues and recommend that rodent tissues intended for metabolomics studies be collected under anesthesia rather than post-euthanasia. |
| Institute | University of Michigan |
| Department | Internal Medicine |
| Laboratory | Evans Lab / Burant Lab |
| Last Name | Evans |
| First Name | Charles |
| Address | 6321 Brehm Tower, 1000 Wall Street, Ann Arbor MI 48105 |
| chevans@umich.edu | |
| Phone | 734-232-8177 |
| Submit Date | 2014-11-21 |
| Num Groups | 6 |
| Total Subjects | 49 |
| Raw Data Available | No |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-02-01 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000203 |
|---|---|
| Analysis type | MS |
| Chromatography type | HILIC |
| Chromatography system | Agilent 1200 |
| Column | Phenomenex Luna NH2 (150 x 2.1mm,3um) |
| MS Type | ESI |
| MS instrument type | TOF |
| MS instrument name | Agilent 6220 TOF |
| Ion Mode | NEGATIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH000135 |
| Chromatography Summary: | Polar metabolites were analyzed by HILIC-MS using an aminopropyl stationary phase |
| Instrument Name: | Agilent 1200 |
| Column Name: | Phenomenex Luna NH2 (150 x 2.1mm,3um) |
| Column Pressure: | 150 bar |
| Column Temperature: | 25 C |
| Flow Gradient: | 80-0% B over 20 minutes, hold 4 minutes, return to 80%B |
| Flow Rate: | 0.25 mL/min |
| Sample Injection: | 25 uL |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 9.9 |
| Solvent B: | 100% acetonitrile |
| Analytical Time: | 32 min |
| Chromatography Type: | HILIC |
