Summary of Study ST000140

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000125. The data can be accessed directly via it's Project DOI: 10.21228/M8DW2B This work is supported by NIH grant, U2C- DK119886.

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Study IDST000140
Study TitleCell Rinsing Solution Comparison
Study TypeCell Rinsing Solution Comparison
Study SummaryAliquots of Jurkat T-lymphocyte cells were washed with 5 different rinsing solutions (0.3% amm. formate, 0.3% amm. acetate, 0.9% NaCl, 1 M PBS, 100 mM PBS) and ran in triplicate to monitor the effect of ion suppression on the electrospray ionization signal.
Institute
University of Florida
DepartmentDept. of Chemistry/SECIM
LaboratoryBiomedical Mass Spectrometry Lab
Last NameUlmer
First NameCandice
AddressP.O. Box 117200, Gainesville, FL 32611
Emailczulmer@chem.ufl.edu
Phone(352) 392-0515
Submit Date2015-03-02
Num Groups5
Total Subjects15
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Uploaded File Size3.1 G
Analysis Type DetailLC-MS
Release Date2016-03-03
Release Version1
Candice Ulmer Candice Ulmer
https://dx.doi.org/10.21228/M8DW2B
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN000222
Analysis type MS
Chromatography type Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 RS
Column ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
MS Type ESI
MS instrument type Orbitrap
MS instrument name Thermo Q Exactive Orbitrap
Ion Mode POSITIVE
Units Peak area

Chromatography:

Chromatography ID:CH000154
Chromatography Summary:Reversed phase
Methods Filename:PFP-metabolomics-pos-350
Instrument Name:Thermo Dionex Ultimate 3000 RS
Column Name:ACE Excel 2 C18-PFP (100 x 2.1mm, 2um)
Column Pressure:800 bar (max)
Column Temperature:30 C
Flow Rate:0.35 mL/min
Injection Temperature:4 C
Sample Injection:5 μL
Solvent A:0.1% formic acid in water
Solvent B:acetonitrile
Analytical Time:18 min
Randomization Order:3,5,7,2,12,10,15,9,13,14,4,8,6,1,11
Chromatography Type:Reversed phase
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