Summary of Study ST000140
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000125. The data can be accessed directly via it's Project DOI: 10.21228/M8DW2B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000140 |
| Study Title | Cell Rinsing Solution Comparison |
| Study Type | Cell Rinsing Solution Comparison |
| Study Summary | Aliquots of Jurkat T-lymphocyte cells were washed with 5 different rinsing solutions (0.3% amm. formate, 0.3% amm. acetate, 0.9% NaCl, 1 M PBS, 100 mM PBS) and ran in triplicate to monitor the effect of ion suppression on the electrospray ionization signal. |
| Institute | University of Florida |
| Department | Dept. of Chemistry/SECIM |
| Laboratory | Biomedical Mass Spectrometry Lab |
| Last Name | Ulmer |
| First Name | Candice |
| Address | P.O. Box 117200, Gainesville, FL 32611 |
| czulmer@chem.ufl.edu | |
| Phone | (352) 392-0515 |
| Submit Date | 2015-03-02 |
| Num Groups | 5 |
| Total Subjects | 15 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Uploaded File Size | 3.1 G |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-03-03 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000222 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 RS |
| Column | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | Peak area |
Chromatography:
| Chromatography ID: | CH000154 |
| Chromatography Summary: | Reversed phase |
| Methods Filename: | PFP-metabolomics-pos-350 |
| Instrument Name: | Thermo Dionex Ultimate 3000 RS |
| Column Name: | ACE Excel 2 C18-PFP (100 x 2.1mm, 2um) |
| Column Pressure: | 800 bar (max) |
| Column Temperature: | 30 C |
| Flow Rate: | 0.35 mL/min |
| Injection Temperature: | 4 C |
| Sample Injection: | 5 μL |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile |
| Analytical Time: | 18 min |
| Randomization Order: | 3,5,7,2,12,10,15,9,13,14,4,8,6,1,11 |
| Chromatography Type: | Reversed phase |
