Summary of Study ST000168
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000146. The data can be accessed directly via it's Project DOI: 10.21228/M83G6X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000168 |
Study Title | Effect of Insulin Sensitizer Therapy on Amino Acids and Their Metabolites |
Study Type | drug + time course |
Study Summary | We previously reported the overall study design for the parent study [29]. The current report primarily examines the effect of three months of insulin sensitizer therapy on plasma concentrations of BCAA, AAA, and AA metabolites in overweight/obese adults with fasting hyperglycemia, defined as either impaired fasting glucose or untreated diabetes [29]. Briefly, 25 drug naïve, Northern European American participants with fasting blood glucose concentrations of 108180 mg/dL were randomized to receive either 45 mg of pioglitazone per day plus 1 g of metformin twice per day (n = 12) or placebo (n = 13) for 12 weeks. We chose metformin based on its proven effect on hepatic insulin sensitivity and pioglitazone based on its effect on peripheral insulin sensitivity. Current use of hypoglycemic medications excluded participants from the present study. |
Institute | Mayo Clinic |
Department | Endocrinology |
Laboratory | Sree Nair |
Last Name | Nair |
First Name | Sreekumaran |
Dasari.Surendra@mayo.edu | |
Submit Date | 2015-05-21 |
Num Groups | 2 |
Total Subjects | 27 |
Raw Data Available | No |
Analysis Type Detail | LC-MS |
Release Date | 2015-06-28 |
Release Version | 1 |
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Combined analysis:
Analysis ID | AN000262 |
---|---|
Analysis type | MS |
Chromatography type | |
Chromatography system | Waters Acquity |
Column | |
MS Type | ESI |
MS instrument type | Triple quadrupole |
MS instrument name | Thermo Quantum Ultra |
Ion Mode | POSITIVE |
Units | uM |
Chromatography:
Chromatography ID: | CH000185 |
Chromatography Summary: | Amino acids in particular are relatively stable for several years, with the exception of their keto-acids (e.g., KIC), which we have not included in the present analysis. Subsequently, we separated the samples using an Acquity UPLC system, followed by mass detection using a TSQ Ultra Quantum from Thermo Finnigan running in ESI positive mode as previously described [32]. We calculated the AA and AA metabolites concentrations using 10 point standard curves as previously described [32]. We handled the pre- and post-intervention samples similarly and analyzed them in the same run. The reproducibility of the UPLC/MS/MS method in the MCRMC is excellent (average CV ~ 9.5%) [32]. |
Instrument Name: | Waters Acquity |