Summary of Study ST000287
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000229. The data can be accessed directly via it's Project DOI: 10.21228/M8259N This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000287 |
| Study Title | Energetics in dystrophic muscle |
| Study Type | Cross-sectional |
| Study Summary | This project utilizes mouse models to establish whether lack of sarcolemma-localized nNOS impairs muscle metabolism and perfusion. |
| Institute | University of Florida |
| Department | SECIM |
| Laboratory | Muscle Physiology Laboratory |
| Last Name | Forbes |
| First Name | Sean |
| Address | University of Florida, College of Public Health & Health Professions, Department of Physical Therapy, Box 100154, UFHSC |
| scforbes@ufl.edu | |
| Phone | 352-273-6111 |
| Submit Date | 2015-09-30 |
| Num Groups | 4 |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2016-12-22 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN000456 | AN000457 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity |
| Column | Thermo Hypercarb (50 x 2.1mm,3um) | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole |
| MS instrument name | Agilent 6490 QQQ | Agilent 6490 QQQ |
| Ion Mode | POSITIVE | POSITIVE |
| Units | pmol/mg dry powder | pmol/mg dry powder |
Chromatography:
| Chromatography ID: | CH000325 |
| Chromatography Summary: | NAD-ATP method |
| Methods Filename: | SB_NAD-ATP_Assay_Agilent.pdf |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Thermo Hypercarb (50 x 2.1mm,3um) |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH000326 |
| Chromatography Summary: | NAD-ATP method |
| Methods Filename: | SB_NADH_Assay_Agilent.pdf |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters Acquity HSS T3 (100 x 2.1mm,1.8um) |
| Column Temperature: | 30 |
| Flow Rate: | 0.54 mL/min |
| Solvent A: | 100% water; 5 mM ammonium acetate, pH 6 |
| Solvent B: | 100% acetonitrile |
| Chromatography Type: | Reversed phase |
