Summary of Study ST000299

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000240. The data can be accessed directly via it's Project DOI: 10.21228/M8N015 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000299
Study TitleConjugated linoleic acid (CLA) study in LDLR-/- mice
Study TypeAcylcarnitine analysis
Study SummaryLDLR-/- mice were fed a high fat high sucrose diet with either 9,11 CLA or 10,12 CLA. Control groups included no supplementation or caloric restriction to mirror weight loss seen in CLA group. Mice were sacrificed and blood was collected into EDTA tubes. Isolated plasma was immediately frozen at -80C. Adipose tissue from gonadal fat (epididymal white adipose tissue, EWAT) and subcutaneous fat (inguinal white adipose tissue, IWAT) and liver were harvested, snap frozen in liquid nitrogen, and immediately frozen at -80C. An aliquot of thawed plasma was prepared for this project and frozen in an eppendorf tube. Small pieces of frozen tissue were cut and weighed on dry ice and packaged in individual foil packets for this project. **Note: tissue weight is approximate**
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2015-02-25
Num Groups4
Total Subjects80
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2016-06-18
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8N015
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN000477
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1260
Column Waters X BridgeC18 (50 x 1mm)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6410A QQQ
Ion Mode POSITIVE
Units uM

Chromatography:

Chromatography ID:CH000340
Methods Filename:QM_004XBridge2mm_ACAR_MRM20150412.m.zip
Instrument Name:Agilent 1260
Column Name:Waters X BridgeC18 (50 x 1mm)
Chromatography Type:Reversed phase
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