Summary of Study ST000468

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000360. The data can be accessed directly via it's Project DOI: 10.21228/M8HK59 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000468
Study TitleEnterococcus faecalis nucleotide profiles following mupirocin or decoyinine
Study TypeExposure to mupirocin or decoyinine
Study SummaryTriplicate samples of E. faecalis OG1RF will be grown in FMC-AUG to OD600 = 0.25. The cultures will be split and exposed to DMSO, mupirocin (0.01mg/mL) or decoyinine (0.1mg/mL) for 15 minutes. 6E9 cells will be harvested by fast filtration, scraped off of membrane into ice-cold PBS, and pelleted by centrifugation, and stored at -80C until shipment.
Institute
University of Florida
DepartmentSECIM
LaboratoryLemos-Abranches
Last NameKajfasz
First NameJessica
Address1395 Center Drive, PO Box 100424 Gainesville, FL 32610
Emailjkajfasz@dental.ufl.edu
Phone352-273-6675
Submit Date2016-09-08
Num Groups3
Total Subjects9
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2017-10-03
Release Version1
Jessica Kajfasz Jessica Kajfasz
https://dx.doi.org/10.21228/M8HK59
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN000731
Analysis type MS
Chromatography type Reversed phase
Chromatography system Agilent 1290 Infinity
Column Thermo Hypercarb (50 x 2.1mm, 3um)
MS Type ESI
MS instrument type Triple quadrupole
MS instrument name Agilent 6490 QQQ
Ion Mode POSITIVE
Units nmol/mg protein

Chromatography:

Chromatography ID:CH000524
Chromatography Summary:NAD-ATP
Methods Filename:SB_NAD-ATP_Assay_Agilent.pdf
Instrument Name:Agilent 1290 Infinity
Column Name:Thermo Hypercarb (50 x 2.1mm, 3um)
Chromatography Type:Reversed phase
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