Summary of Study ST000753

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000543. The data can be accessed directly via it's Project DOI: 10.21228/M8GH3J This work is supported by NIH grant, U2C- DK119886.

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Study IDST000753
Study TitleComparison of the metabolome and lipidome of wild type and mdx/mTR mice
Study TypeMS analysis
Study SummaryThere are two groups of mice in the study, wild type mice (WT) and mdx/mTR mice (mdx or mdx/mTR) that are a model of musclar dystrophy. We collected the tibialis anterior muscles from these mice (N=6 in each group), and would like to perform TCA-plus analysis and shotgun lipidomics analysis on the mice, and compare across the two groups.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-06-19
Num Groups2
Total Subjects12
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2017-12-06
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8GH3J
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN001181 AN001182 AN001183
Analysis type MS MS MS
Chromatography type Reversed phase Reversed phase HILIC
Chromatography system Shimadzu CTO-20A Nexera X2 Shimadzu CTO-20A Nexera X2 Agilent
Column Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Waters Acquity HSS T3 (50 x 2.1mm,1.8um) Phenomenex Luna NH2 (150 x 1mm,3um)
MS Type ESI ESI ESI
MS instrument type Triple TOF Triple TOF QTOF
MS instrument name ABI Sciex 5600+ TripleTOF ABI Sciex 5600+ TripleTOF Agilent 6520B QTOF
Ion Mode NEGATIVE POSITIVE NEGATIVE
Units peak area normalized peak area normalized counts

Chromatography:

Chromatography ID:CH000829
Instrument Name:Shimadzu CTO-20A Nexera X2
Column Name:Waters Acquity HSS T3 (50 x 2.1mm,1.8um)
Column Temperature:55
Flow Rate:0.400uL/min
Solvent A:40% acetonitrile/60% water; 10 mM ammonium acetate
Solvent B:10% acetonitrile/5% water/85% isopropanol; 10 mM ammonium acetate
Chromatography Type:Reversed phase
  
Chromatography ID:CH000830
Instrument Name:Agilent
Column Name:Phenomenex Luna NH2 (150 x 1mm,3um)
Flow Rate:0.075 mL/min
Solvent A:100% water; 5 mM ammonium acetate, pH 9.9
Solvent B:100% acetonitrile
Chromatography Type:HILIC
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