Summary of Study ST000836

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000596. The data can be accessed directly via it's Project DOI: 10.21228/M8MX15 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000836
Study TitlePilot Human Muscle oral glucose tolerance test (OGTT)
Study TypeMS analysis
Study SummaryObesity is associated with metabolic inflexibility, the inability to switch from lipid to glucose oxidation during physiological conditions favoring excess glucose and insulin, which could contribute to the increased incidence of type 2 diabetes. Despite this knowledge, the role of skeletal muscle metabolites during these physiological conditions remain unclear and warrant further investigation. The purpose of this pilot study is to determine if: 1) there are differences in skeletal muscle glycolysis and TCA metabolites and amino acids between lean and obese individuals in the fasted state, and 2) if these metabolites change after 50 min of glucose ingestion, and 3) if there are differences in these metabolites between lean and obese individuals after 50 min of glucose ingestion.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2017-08-02
Num Groups5
Total Subjects6
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2018-02-07
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8MX15
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN001338 AN001339
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Agilent Agilent
Column Phenomenex Luna NH2 (150 x 1mm,3um) Phenomenex Luna NH2 (150 x 1mm,3um)
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6520B QTOF Agilent 6520B QTOF
Ion Mode NEGATIVE NEGATIVE
Units ISTD response ratio nmol/mg

Chromatography:

Chromatography ID:CH000935
Instrument Name:Agilent
Column Name:Phenomenex Luna NH2 (150 x 1mm,3um)
Solvent A:100% water; 5 mM ammonium acetate, pH 9.9
Solvent B:100% acetonitrile
Chromatography Type:HILIC
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