Summary of Study ST001032

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000690. The data can be accessed directly via it's Project DOI: 10.21228/M8GQ3M This work is supported by NIH grant, U2C- DK119886.

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Study IDST001032
Study TitleSingle-cell Profiling of Cationic and Anionic Metabolites in Live Frog (Xenopus) Embryos using Microprobe Capillary Electrophoresis Mass Spectrometry
Study TypeMetabolic profiling of single cells
Study SummaryThe goal of this study was to enable the analysis of anionic and cationic metabolites from the same identified single cell in Xenopus laevis embryos. A 10 nL portion of identified animal-ventral (V1) cells was aspirated from 8-cell embryos, and metabolites were extracted from the aspirate, before characterization of cationic and anionic compounds using a custom-built capillary electrophoresis (CE) electrospray ionization (ESI) mass spectrometry platform. A total of ~250 cationic molecular features and ~150 anionic molecular features were detected, including 76 metabolites that were identified in this study. Pathway analysis of the identified metabolites highlighted arginine-proline metabolism of significance.
Institute
University of Maryland
DepartmentDepartment of Chemistry & Biochemistry
LaboratoryNemes Laboratory
Last NameNemes
First NamePeter
Address0107 Chemistry Building 8051 Regents Drive
Emailnemes@umd.edu
Phone3014050373
Submit Date2018-08-08
Num Groups4 biological replicates (each different cell from a different embryo) + 1-to-2 technical replicates (same extract analyzed multiple times)
Total Subjects4 different V1 cells were analyzed, each from a different embryo
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2019-09-23
Release Version1
Peter Nemes Peter Nemes
https://dx.doi.org/10.21228/M8GQ3M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN001692 AN001693
Analysis type MS MS
Chromatography type CE CE
Chromatography system Custom-built CE system Custom-built CE system
Column Bare fused silica capillary Bare fused silica capillary
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Bruker Impact HD Bruker Impact HD
Ion Mode POSITIVE NEGATIVE
Units peak area peak area

Chromatography:

Chromatography ID:CH001191
Chromatography Summary:Metabolites were separated in a custom-built capillary electrophoresis (CE) system.
Instrument Name:Custom-built CE system
Column Name:Bare fused silica capillary
Column Temperature:Room temperature
Injection Temperature:Room temperature
Sample Injection:Ca. 10 nL
Solvent A:During cationic separation, the background electrolyte used in postive ionization mode was 1% formic acid in water (isocratic).
Analytical Time:45 min of separation
Capillary Voltage:During cationic separation, +19,000-20,000 V was applied on the inlet end of the CE capillary.
Preconditioning:Sodium hydroxide solution
Sheath Liquid:During cationic analysis, the electrospray sheath solution was 50% methanol with 0.1% formic acid.
Chromatography Type:CE
  
Chromatography ID:CH001192
Chromatography Summary:Metabolites were separated in a custom-built capillary electrophoresis (CE) system.
Instrument Name:Custom-built CE system
Column Name:Bare fused silica capillary
Column Temperature:Room temperature
Injection Temperature:Room temperature
Sample Injection:Ca. 10 nL
Solvent A:During anionic separation, the background electrolyte used in postive ionization mode was 20 mM ammonium bicarbonate (isocratic).
Analytical Time:45 min of separation
Capillary Voltage:During anionic, +19,000-20,000 V was applied on the inlet end of the CE capillary.
Preconditioning:Sodium hydroxide solution
Sheath Liquid:During cationic analysis, the electrospray sheath solution was 0.2 mM ammonium bicarbonate in 50% isopropanol.
Chromatography Type:CE
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