Summary of Study ST001140
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000761. The data can be accessed directly via it's Project DOI: 10.21228/M89Q32 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001140 |
| Study Title | Changes in the Canine Plasma Lipidome after Short- and Long-Term Excess Glucocorticoid Exposure |
| Study Summary | Glucocorticoids (GCs) are widely used in veterinary and human medicine. Chromic endogenous or iatrogenic GC overexposure impairs metabolic function and can result in diverse side-effects, including Cushing’s syndrome. This study examines the effects of experimentally induced short-term and long-term GC excess (induced by prednisolone and tetracosactide, respectively) on the plasma lipidome of Beale dogs. Both, long- and short-term GC resulted in significant changes of the plasma lipidome. |
| Institute | National University of Singapore;University of Zurich |
| Department | Singapore Lipidomics Incubator (SLING);Vetsuisse Faculty, University of Zurich |
| Laboratory | Singapore Lipidomics Incubator (SLING), National University of Singapore |
| Last Name | Burla |
| First Name | Bo |
| Address | 28 Medical Drive, Singapore 117456, Singapore |
| bo.burla@nus.edu.sg | |
| Phone | +6565166683 |
| Submit Date | 2019-01-19 |
| Num Groups | 2 |
| Total Subjects | 14 |
| Num Males | 9 |
| Num Females | 5 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-03-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN001870 | AN001871 | AN001872 | AN001873 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | Reversed phase | Reversed phase | HILIC | Reversed phase |
| Chromatography system | Agilent 1290 Infinity | Agilent 1290 Infinity | Agilent 1290 Infinity | Agilent 1100 |
| Column | Agilent Zorbax RRHD Eclipse Plus C18 (50 x 2.1mm,1.8um,95 Å) | Agilent Zorbax RRHD Eclipse Plus C18 (50 x 2.1mm,1.8um,95 Å) | Waters Acquity BEH HILIC (100 x 2.1mm,1.7um,130 Å) | Agilent Zorbax Eclipse XDB C18 (150 x 3.0mm,1.8um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Triple quadrupole | Triple quadrupole | Triple quadrupole | Triple quadrupole |
| MS instrument name | Agilent 6460 QQQ | Agilent 6495 QQQ | Agilent 6490 QQQ | ABI Sciex 4000 QTrap |
| Ion Mode | POSITIVE | POSITIVE | POSITIVE | POSITIVE |
| Units | µmol/L | µmol/L | µmol/L | µmol/L |
Chromatography:
| Chromatography ID: | CH001351 |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Agilent Zorbax RRHD Eclipse Plus C18 (50 x 2.1mm,1.8um,95 Å) |
| Column Temperature: | 40 |
| Flow Gradient: | 20% B: 2 min, 20% - 60% B: 2 - 7 min, 60% - 100% B: 7 - 9 min, 20% B: 9 - 10.8 min |
| Flow Rate: | 0.4 mL/min |
| Sample Injection: | 2 µL |
| Solvent A: | 60% acetonitrile/40% water; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001352 |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Agilent Zorbax RRHD Eclipse Plus C18 (50 x 2.1mm,1.8um,95 Å) |
| Column Temperature: | 40 |
| Flow Gradient: | 20% B: 2 min, 20% - 60% B: 2 - 7 min, 60% - 100% B: 7 - 9 min, 20% B: 9 - 10.8 min |
| Flow Rate: | 0.4 mL/min |
| Sample Injection: | 1 µL |
| Solvent A: | 60% acetonitrile/40% water; 10 mM ammonium formate |
| Solvent B: | 90% isopropanol/10% acetonitrile; 10 mM ammonium formate |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH001353 |
| Instrument Name: | Agilent 1290 Infinity |
| Column Name: | Waters Acquity BEH HILIC (100 x 2.1mm,1.7um,130 Å) |
| Column Temperature: | 60 |
| Flow Gradient: | 99.9% B: 0 - 5 min; 40% B: 5 - 5.5 min; 10% B: 5.5 - 6.6 min and 99.9% B: 6.6 - 9.6 min (total run time 9.6 min) |
| Flow Rate: | 0.4 mL/min |
| Sample Injection: | 2 µL |
| Solvent A: | 50% water/50% acetonitrile; 25 mM ammonium formate, pH 4.6 |
| Solvent B: | 95% acetonitrile/5% water; 25 mM ammonium formate, pH 4.6 |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH001354 |
| Instrument Name: | Agilent 1100 |
| Column Name: | Agilent Zorbax Eclipse XDB C18 (150 x 3.0mm,1.8um) |
| Column Temperature: | 25 |
| Flow Gradient: | Isocratic (25 min) |
| Flow Rate: | 0.128 mL/min |
| Sample Injection: | 10 µL |
| Solvent A: | 50% chloroform/50% methanol; 2 mM ammonium acetate |
| Chromatography Type: | Reversed phase |
