Summary of Study ST001182

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000794. The data can be accessed directly via it's Project DOI: 10.21228/M8240W This work is supported by NIH grant, U2C- DK119886.

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Study IDST001182
Study TitleCorrelations between LC-MS/MS-detected Glycomics and NMR-detected Metabolomics in Caenorhabditis (part -I) elegans Development.
Study SummaryThis study examines the relationship between glycans, metabolites, and development in C. elegans. Samples of N2 animals were synchronized and grown to five different time points that ranged from L1 to a mixed population of adults, gravid adults, and offspring.
Institute
University of Georgia
DepartmentComplex Carbohydrate Research Center
LaboratoryEdison/ Wells
Last NameEdison
First NameArthur
Address315 Riverbend Road Athens, Georgia 30602-4712 USA
Emailaedison@uga.edu
Phone706-542-8156
Submit Date2019-05-14
Raw Data AvailableYes
Analysis Type DetailMS(Dir. Inf.)
Release Date2019-07-17
Release Version1
Arthur Edison Arthur Edison
https://dx.doi.org/10.21228/M8240W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN001960
Analysis type MS
Chromatography type None (Direct infusion)
Chromatography system none
Column none
MS Type ESI
MS instrument type Ion trap
MS instrument name Thermo Velos Pro Linear Ion Trap
Ion Mode POSITIVE
Units peak area

Chromatography:

Chromatography ID:CH001421
Instrument Name:none
Column Name:none
Column Pressure:120 bar
Column Temperature:60°C
Flow Gradient:linear gradient from 30% to 70% LC-MS Buffer B
Flow Rate:300 nL/min
Injection Temperature:RT
Internal Standard:13C-Permethylated isomaltopentaose (DP5)
Sample Injection:5 µL
Solvent A:100% water; 0.02% acetic acid; 1 mM LiOAc
Solvent B:80% acetonitrile/20% water; 0.02% acetic acid; 1 mM lithium acetate
Analytical Time:180 min
Oven Temperature:60 °C
Chromatography Type:None (Direct infusion)
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