Summary of Study ST001287

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000869. The data can be accessed directly via it's Project DOI: 10.21228/M8C40P This work is supported by NIH grant, U2C- DK119886.

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Study IDST001287
Study TitleLuminal succinate in UC-HMA (human microbiota-associated) mice.
Study TypeMS analysis
Study SummaryPatients with ulcerative colitis (UC) are known to be at higher risk for Clostridium difficile (C. difficile) infection (CDI), and CDI in UC patients is recognized as a major clinical problem because it worsens UC outcome. In order to assess the role of gut dysbiosis, seen in UC patients, we have established a human microbiota-associated mouse model in which germ-free mice are colonized with gut microbiota from UC patients. Utilizing this model, we found that UC microbiota colonized HMA mice (UC-HMA mice) were susceptible to CDI. To address the mechanism by which UC-HMA mice are unable to acquire the colonization resistance against C. difficile, we analyzed the luminal metabolites in UC-HMA mice, especially in succinate which is a crucial metabolite doe the growth of C. difficile.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
AddressAnn Arbor, MI
Emailmkachman@med.umich.edu
Phone734-232-0842
Submit Date2019-12-11
Num Groups63
Total Subjects3
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2020-01-13
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8C40P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN002147 AN002148
Analysis type MS MS
Chromatography type HILIC HILIC
Chromatography system Agilent Q-TOF 6520 Agilent Q-TOF 6520
Column Phenomenex Luna NH2, 1mm ID Phenomenex Luna NH2, 1mm ID
MS Type ESI ESI
MS instrument type QTOF QTOF
MS instrument name Agilent 6520 QTOF Agilent 6520 QTOF
Ion Mode NEGATIVE NEGATIVE
Units pmol/mg Peak area normalized

Chromatography:

Chromatography ID:CH001571
Methods Filename:002-HILIC-27min-1mm-flatInsert_-_A2B2.m.zip
Instrument Name:Agilent Q-TOF 6520
Column Name:Phenomenex Luna NH2, 1mm ID
Chromatography Type:HILIC
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