Summary of Study ST001359
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000929. The data can be accessed directly via it's Project DOI: 10.21228/M8M98M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001359 |
| Study Title | Monophasic lipidomics extraction in cancer cell line |
| Study Type | Lipidomics |
| Study Summary | We performed a comprehensive characterization of a monophasic extraction method in cancer cell lines. We used pharmacological perturbation on HepG2 cells to identify changes in different lipid families. We optimized the MS parameters, the chromatography and the data analysis to perform rapid and robust lipidomics analysis from cell lines. |
| Institute | Beatson Institute for Cancer Research |
| Department | Metabolomics |
| Laboratory | Metabolomics |
| Last Name | Rodriguez Blanco |
| First Name | Giovanny |
| Address | Garscube State, Switchback road, Glasgow |
| g.blanco@ed.ac.uk | |
| Phone | +447526056849 |
| Submit Date | 2020-03-26 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-09-29 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN002263 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Ultimate 3000 |
| Column | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Orbitrap |
| MS instrument name | Thermo Q Exactive Orbitrap |
| Ion Mode | POSITIVE |
| Units | Normalised peak area |
Chromatography:
| Chromatography ID: | CH001662 |
| Chromatography Summary: | The mobile phases consisted of 60:40 ACN: H2O with 10mM ammonium formate, 0.1% formic acid and 5µM of phosphoric acid (A) and 90:10 IPA:ACN with 10 mM ammonium formate, 0.1% formic acid and 5µM phosphoric acid (B). The gradient was as follows: 0-2 min 30% (B); 2-8 min 50% (B); 8-15 min 99% (B), 15-16 min 99% (B), 16-17 min 30% (B). Sample temperature was maintained at 6°C in the autosampler and 5 µL of sample were injected into the LC-MS instrument. |
| Instrument Name: | Ultimate 3000 |
| Column Name: | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
| Flow Gradient: | 0-2 min 30% (B); 2-8 min 50% (B); 8-15 min 99% (B), 15-16 min 99% (B), 16-17 min 30% (B). |
| Solvent A: | 60% acetonitrile/40% water; 0.1% formic acid; 10mM ammonium formate; 5µM phosphoric acid |
| Solvent B: | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate;5µM phosphoric acid |
| Chromatography Type: | Reversed phase |
