Summary of Study ST001491

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001009. The data can be accessed directly via it's Project DOI: 10.21228/M88H6T This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001491
Study TitleGlobal Urine Metabolic Profiling to Predict Gestational Age in Term and Preterm Pregnancies
Study SummaryAssessment of gestational age (GA) is key to provide optimal care during pregnancy. However, its accurate determination remains challenging in low- and middle-resource countries, where access to obstetric ultrasound is limited. Hence, there is an urgent need to develop clinical approaches that allow accurate and inexpensive estimation of GA. We investigated the ability of urinary metabolites to predict GA at time of collection in a diverse multi-site cohort (n = 99) using a broad-spectrum liquid chromatography coupled with mass spectrometry (LC-MS) platform. Our approach detected a myriad of steroid hormones and their derivatives including estrogens, progesterones, corticosteroids and androgens that associated with pregnancy progression. We developed a prediction model that predicted GA with high accuracy using the levels of three metabolites (rho = 0.87, .RMSE = 1.58 weeks). These predictions were robust irrespective of whether the pregnancy went to term or ended prematurely. Overall, we demonstrate the feasibility of implementing urine collection for metabolomics analysis in large-scale multi-site studies and we report a predictive model of GA with a potential clinical value.
Institute
Stanford University
Last NameContrepois
First NameKevin
Address300 Pasteur Dr
Emailkcontrep@stanford.edu
Phone6506664538
Submit Date2020-09-27
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2022-05-16
Release Version1
Kevin Contrepois Kevin Contrepois
https://dx.doi.org/10.21228/M88H6T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN002470 AN002471 AN002472 AN002473
Analysis type MS MS MS MS
Chromatography type HILIC HILIC Reversed phase Reversed phase
Chromatography system Thermo Dionex Ultimate 3000 RS Thermo Dionex Ultimate 3000 RS Thermo Dionex Ultimate 3000 RS Thermo Dionex Ultimate 3000 RS
Column SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) Hypersil GOLD (150 x 2.1mm,1.9um) Hypersil GOLD (150 x 2.1mm,1.9um)
MS Type ESI ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Q Exactive HF hybrid Orbitrap Thermo Q Exactive HF hybrid Orbitrap Thermo Q Exactive HF hybrid Orbitrap Thermo Q Exactive HF hybrid Orbitrap
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units MS count MS Counts MS Counts MS Counts

Chromatography:

Chromatography ID:CH001810
Chromatography Summary:HILIC experiments were performed using a ZIC-HILIC column 2.1x100 mm, 3.5μm, 200Å (Merck Millipore) and mobile phase solvents consisting of 10mM ammonium acetate in 50/50 acetonitrile/water (A) and 10 mM ammonium acetate in 95/5 acetonitrile/water (B).(Contrepois et al., 2015)
Instrument Name:Thermo Dionex Ultimate 3000 RS
Column Name:SeQuant ZIC-HILIC (100 x 2.1mm,3.5um)
Column Temperature:40
Flow Rate:0.5 ml/min
Solvent A:95% acetonitrile/5% water; 10 mM ammonium acetate
Solvent B:95% acetonitrile/5% water; 10 mM ammonium acetate
Chromatography Type:HILIC
  
Chromatography ID:CH001811
Chromatography Summary:RPLC experiments were performed using a Hypersil GOLD column 2.1 x 150 mm, 1.9 µm, 175Å (Thermo Scientific) and mobile phase solvents consisting of 0.06% acetic acid in water (A) and 0.06% acetic acid in methanol (B). (Contrepois et al., 2015)
Chromatography Comments:Hypersil GOLD column 2.1 x 150 mm, 1.9 µm, 175Å (Thermo Scientific)
Instrument Name:Thermo Dionex Ultimate 3000 RS
Column Name:Hypersil GOLD (150 x 2.1mm,1.9um)
Column Temperature:60
Flow Rate:0.6 ml/min
Solvent A:100% water; 0.06% acetic acid
Solvent B:100% methanol; 0.06% acetic acid
Chromatography Type:Reversed phase
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