Summary of Study ST001670

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001073. The data can be accessed directly via it's Project DOI: 10.21228/M80Q4N This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST001670
Study Title	Metabolomics characterization of zebrafish larvae
Study Type	MS analysis
Study Summary	Four different treatment groups were used for metabolite characterization: 5 dpf larvae with/without beta-cell ablation and with/without folinic acid treatment.
Institute	North Carolina State University
Department	Molecular and Structural Biochemistry
Last Name	Duan
First Name	Likun
Address	120 W Broughton Dr.
Email	lduan2@ncsu.edu
Phone	9195939853
Submit Date	2021-01-15
Raw Data Available	Yes
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2021-03-15
Release Version	1

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID	AN002725	AN002726
Analysis type	MS	MS
Chromatography type	HILIC	Reversed phase
Chromatography system	Vanquish UHPLC (Thermo Fisher Scientific).	Thermo Vanquish UHPLC
Column	Waters Xbridge amide HILIC (100 x 2.1mm,3.5um)	Luna C18 LC (150 x 4.6 mm,3um)
MS Type	ESI	ESI
MS instrument type	Ion trap	Ion trap
MS instrument name	Thermo Orbitrap Exploris 480	Thermo Orbitrap Exploris 480
Ion Mode	UNSPECIFIED	UNSPECIFIED
Units	peak area	peak area

Chromatography:

Chromatography ID:	CH002011
Chromatography Summary:	For this polar metabolite analysis, a hydrophilic interaction chromatography method (HILIC) with an Xbridge amide column (100 x 2.1 mm i.d., 3.5 µm; Waters) was used for compound separation at 25 °C. Mobile phase A: water with 5 mM ammonium acetate (pH 6.8), and mobile phase B: 100 % acetonitrile. Linear gradient was: 0 min, 85% B; 1.5 min, 85% B; 5.5 min, 35% B; 6.9 min, 35% B; 10.5 min, 35% B; 10.6 min, 10% B; 12.5 min, 10% B; 13.5 min, 85% B; 17.9 min, 85% B; 18 min, 85% B; 20 min, 85% B. The flow rate was: 0-5.5 min, 0.11 ml/min; 6.9-10.5 min, 0.13 ml/min; 10.6-17.9 min, 0.25 ml/min; 18-20 min, 0.11 ml/min.
Instrument Name:	Vanquish UHPLC (Thermo Fisher Scientific).
Column Name:	Waters Xbridge amide HILIC (100 x 2.1mm,3.5um)
Column Temperature:	25
Flow Gradient:	0 min, 85% B; 1.5 min, 85% B; 5.5 min, 35% B; 6.9 min, 35% B; 10.5 min, 35% B; 10.6 min, 10% B; 12.5 min, 10% B; 13.5 min, 85% B; 17.9 min, 85% B; 18 min, 85% B; 20 min, 85% B.
Flow Rate:	0-5.5 min, 0.11 ml/min; 6.9-10.5 min, 0.13 ml/min; 10.6-17.9 min, 0.25 ml/min; 18-20 min, 0.11 ml/min
Solvent A:	100% water; 5 mM ammonium acetate, pH 6.8
Solvent B:	100% acetonitrile
Chromatography Type:	HILIC

Chromatography ID:	CH002012
Chromatography Summary:	The analysis of folic acid and its derivatives was performed using Luna column (100 x 3 mm i.d., 3 µm, C18(2), 100 Å, Phenomenex) at 25 °C. Mobile phase A: water with 0.1% formic acid, and mobile phase B: 100 % methanol. Linear gradient was: 0 min, 2% B; 1.5 min, 2% B; 3.0 min, 15% B; 5.5 min, 95% B; 14.5 min, 95% B; 15.0 min, 2% B; 22.0 min, 2% B. The flow rate was 0.150 ml/min.
Chromatography Comments:	Luna® 3 µm C18(2) 100 Å, LC Column 150 x 4.6 mm, Ea
Instrument Name:	Thermo Vanquish UHPLC
Column Name:	Luna C18 LC (150 x 4.6 mm,3um)
Column Temperature:	25
Flow Gradient:	0 min, 2% B; 1.5 min, 2% B; 3.0 min, 15% B; 5.5 min, 95% B; 14.5 min, 95% B; 15.0 min, 2% B; 22.0 min, 2% B.
Flow Rate:	0.150 ml/min
Solvent A:	100% water; 0.1% formic acid
Solvent B:	100% methanol
Chromatography Type:	Reversed phase