Summary of Study ST002112
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001338. The data can be accessed directly via it's Project DOI: 10.21228/M8RX2S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002112 |
Study Title | Global, distinctive and personal changes in molecular and microbial profiles induced by specific fibers in humans (Untargeted) |
Study Summary | Dietary fibers act through the microbiome and improve cardiovascular health, metabolic disorders and cancer prevention. To understand health benefits of dietary fiber supplementation we investigated two popular purified fibers, arabinoxylan (AX) and long-chain inulin (LCI), and a mixture of five fibers. We present multi-omic signatures of metabolomics, lipidomics, proteomics, metagenomics, a cytokine panel and clinical measurements on healthy and insulin resistant participants. Each fiber is associated with fiber-dependent biochemical and microbial responses. AX consumption associates with a significant reduction in LDL and an increase in bile acids, contributing to its observed cholesterol reduction. LCI is associated with an increase in Bifidobacterium. However, at the highest LCI dose there is increased inflammation and elevation in the liver enzyme alanine aminotransferase. This study yields insights into the effects of fiber supplementation, it provides insights into mechanisms behind fiber induced cholesterol reduction, and it shows effects of individual, purified fibers on the microbiome. |
Institute | Stanford University |
Last Name | Lancaster |
First Name | Samuel |
Address | 240 Pasteur Dr, BMI bldg 4400, Stanford California, 94305 |
slancast@stanford.edu | |
Phone | 6126004033 |
Submit Date | 2022-05-06 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2023-03-13 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004115 | AN004116 | AN004117 | AN004118 |
---|---|---|---|---|
Analysis type | MS | MS | MS | MS |
Chromatography type | HILIC | HILIC | Reversed phase | Reversed phase |
Chromatography system | Thermo Vanquish | Thermo Vanquish | Thermo Dionex Ultimate 3000 RS | Thermo Dionex Ultimate 3000 RS |
Column | SeQuant ZIC-HILIC (100 x 2.1mm, 3.5um) | SeQuant ZIC-HILIC (100 x 2.1mm, 3.5um) | Agilent Zorbax SBaq (2.1 x 50 mm, 1.8 um) | Agilent Zorbax SBaq (2.1 x 50 mm, 1.8 um) |
MS Type | ESI | ESI | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
MS instrument name | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Orbitrap | Thermo Q Exactive Orbitrap |
Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
Units | Area | Area | Area | Area |
Chromatography:
Chromatography ID: | CH003049 |
Chromatography Summary: | HILIC experiments were performed using a ZIC-HILIC column 2.1x100 mm, 3.5μm, 200Å (Merck Millipore) and mobile phase solvents consisting of 10mM ammonium acetate in 50/50 acetonitrile/water (A) and 10 mM ammonium acetate in 95/5 acetonitrile/water (B).(Contrepois et al., 2015) |
Instrument Name: | Thermo Vanquish |
Column Name: | SeQuant ZIC-HILIC (100 x 2.1mm, 3.5um) |
Column Temperature: | 40 |
Flow Gradient: | The gradient profile used was 99%B for 2 minutes, 99-1% B over 15 minutes, 1%B for 3 minutes. 99%B for 5 minutes. |
Flow Rate: | .5 ml/min |
Solvent A: | 10mM ammonium acetate in 50/50 acetonitrile/water |
Solvent B: | 10 mM ammonium acetate in 95/5 acetonitrile/water |
Chromatography Type: | HILIC |
Chromatography ID: | CH003050 |
Chromatography Summary: | RPLC experiments were performed using a Zorbax SBaq column 2.1 x 50 mm, 1.8 μm, 100Å (Agilent Technologies) and mobile phase solvents consisting of 0.06% acetic acid in water (A) and 0.06% acetic acid in methanol (B). (Contrepois et al., 2015) |
Instrument Name: | Thermo Dionex Ultimate 3000 RS |
Column Name: | Agilent Zorbax SBaq (2.1 x 50 mm, 1.8 um) |
Column Temperature: | 60 |
Flow Gradient: | The gradient profile used was 1% B for 1 minute, 1%-80% B over 8 minutes, 80% B for 3 minutes, 1% B for 5 minutes. |
Flow Rate: | .6ml/min |
Solvent A: | 0.06% acetic acid in water |
Solvent B: | 0.06% acetic acid in methanol |
Chromatography Type: | Reversed phase |