Summary of Study ST002112

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001338. The data can be accessed directly via it's Project DOI: 10.21228/M8RX2S This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002112
Study TitleGlobal, distinctive and personal changes in molecular and microbial profiles induced by specific fibers in humans (Untargeted)
Study SummaryDietary fibers act through the microbiome and improve cardiovascular health, metabolic disorders and cancer prevention. To understand health benefits of dietary fiber supplementation we investigated two popular purified fibers, arabinoxylan (AX) and long-chain inulin (LCI), and a mixture of five fibers. We present multi-omic signatures of metabolomics, lipidomics, proteomics, metagenomics, a cytokine panel and clinical measurements on healthy and insulin resistant participants. Each fiber is associated with fiber-dependent biochemical and microbial responses. AX consumption associates with a significant reduction in LDL and an increase in bile acids, contributing to its observed cholesterol reduction. LCI is associated with an increase in Bifidobacterium. However, at the highest LCI dose there is increased inflammation and elevation in the liver enzyme alanine aminotransferase. This study yields insights into the effects of fiber supplementation, it provides insights into mechanisms behind fiber induced cholesterol reduction, and it shows effects of individual, purified fibers on the microbiome.
Institute
Stanford University
Last NameLancaster
First NameSamuel
Address240 Pasteur Dr, BMI bldg 4400, Stanford California, 94305
Emailslancast@stanford.edu
Phone6126004033
Submit Date2022-05-06
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2023-03-13
Release Version1
Samuel Lancaster Samuel Lancaster
https://dx.doi.org/10.21228/M8RX2S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN004115 AN004116 AN004117 AN004118
Analysis type MS MS MS MS
Chromatography type HILIC HILIC Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish Thermo Dionex Ultimate 3000 RS Thermo Dionex Ultimate 3000 RS
Column SeQuant ZIC-HILIC (100 x 2.1mm, 3.5um) SeQuant ZIC-HILIC (100 x 2.1mm, 3.5um) Agilent Zorbax SBaq (2.1 x 50 mm, 1.8 um) Agilent Zorbax SBaq (2.1 x 50 mm, 1.8 um)
MS Type ESI ESI ESI ESI
MS instrument type Orbitrap Orbitrap Orbitrap Orbitrap
MS instrument name Thermo Q Exactive Plus Orbitrap Thermo Q Exactive Plus Orbitrap Thermo Q Exactive Orbitrap Thermo Q Exactive Orbitrap
Ion Mode POSITIVE NEGATIVE POSITIVE NEGATIVE
Units Area Area Area Area

Chromatography:

Chromatography ID:CH003049
Chromatography Summary:HILIC experiments were performed using a ZIC-HILIC column 2.1x100 mm, 3.5μm, 200Å (Merck Millipore) and mobile phase solvents consisting of 10mM ammonium acetate in 50/50 acetonitrile/water (A) and 10 mM ammonium acetate in 95/5 acetonitrile/water (B).(Contrepois et al., 2015)
Instrument Name:Thermo Vanquish
Column Name:SeQuant ZIC-HILIC (100 x 2.1mm, 3.5um)
Column Temperature:40
Flow Gradient:The gradient profile used was 99%B for 2 minutes, 99-1% B over 15 minutes, 1%B for 3 minutes. 99%B for 5 minutes.
Flow Rate:.5 ml/min
Solvent A:10mM ammonium acetate in 50/50 acetonitrile/water
Solvent B:10 mM ammonium acetate in 95/5 acetonitrile/water
Chromatography Type:HILIC
  
Chromatography ID:CH003050
Chromatography Summary:RPLC experiments were performed using a Zorbax SBaq column 2.1 x 50 mm, 1.8 μm, 100Å (Agilent Technologies) and mobile phase solvents consisting of 0.06% acetic acid in water (A) and 0.06% acetic acid in methanol (B). (Contrepois et al., 2015)
Instrument Name:Thermo Dionex Ultimate 3000 RS
Column Name:Agilent Zorbax SBaq (2.1 x 50 mm, 1.8 um)
Column Temperature:60
Flow Gradient:The gradient profile used was 1% B for 1 minute, 1%-80% B over 8 minutes, 80% B for 3 minutes, 1% B for 5 minutes.
Flow Rate:.6ml/min
Solvent A:0.06% acetic acid in water
Solvent B:0.06% acetic acid in methanol
Chromatography Type:Reversed phase
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