Summary of Study ST002539
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001635. The data can be accessed directly via it's Project DOI: 10.21228/M8C42F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST002539 |
| Study Title | Microbial metabolomic responses to changes in temperature and salinity along the western Antarctic Peninsula. |
| Study Type | Study of particulate metabolites in phytoplankton and sea-ice algae along the Western Antarctic Peninsula |
| Study Summary | Seasonal cycles within the marginal ice zones in polar regions include large shifts in temperature and salinity that strongly influence microbial abundance and physiology. However, the combined effects of concurrent temperature and salinity change on microbial community structure and biochemical composition during transitions between seawater and sea ice are not well understood. Coastal marine communities along the western Antarctic Peninsula were sampled and surface seawater was incubated at combinations of temperature and salinity mimicking the formation (cold, salty) and melting (warm, fresh) of sea ice to evaluate how these factors may shape community composition and particulate metabolite pools during seasonal transitions. Bacterial and algal community structures were tightly coupled to each other and distinct across sea-ice, seawater, and sea-ice-meltwater field samples, with unique metabolite profiles in each habitat. During short-term (approximately 10-day) incubations of seawater microbial communities under different temperature and salinity conditions, community compositions changed minimally while metabolite pools shifted greatly, strongly accumulating compatible solutes like proline and glycine betaine under cold and salty conditions. Lower salinities reduced total metabolite concentrations in particulate matter, which may indicate a release of metabolites into the labile dissolved organic matter pool. Low salinity also increased acylcarnitine concentrations in particulate matter, suggesting a potential for fatty acid degradation and reduced nutritional value at the base of the food web during freshening. Our findings have consequences for food web dynamics, microbial interactions, and carbon cycling as polar regions undergo rapid climate change. |
| Institute | University of Washington, School of Oceanography |
| Department | School of Oceanography |
| Laboratory | Young Lab |
| Last Name | Dawson |
| First Name | Hannah |
| Address | 1501 NE Boat St, Seattle, WA, 98195, USA |
| hmdawson@uw.edu | |
| Phone | 5404547754 |
| Submit Date | 2023-03-27 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | LC-MS |
| Release Date | 2023-05-25 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN004181 | AN004182 | AN004183 | AN004184 |
|---|---|---|---|---|
| Analysis type | MS | MS | MS | MS |
| Chromatography type | HILIC | HILIC | Reversed phase | Reversed phase |
| Chromatography system | Waters Acquity I-Class | Waters Acquity I-Class | Waters Acquity I-Class | Waters Acquity I-Class |
| Column | SeQuant ZIC- pHILIC (150 x 2.1mm,5um) | SeQuant ZIC- pHILIC (150 x 2.1mm,5um) | Waters ACQUITY UPLC CSH C18 (150 x 2.1mm,1.7um) | Waters ACQUITY UPLC HSS CN (100 x 2.1mm,1.8um) |
| MS Type | ESI | ESI | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE | POSITIVE | NEGATIVE |
| Units | Estimated metabolite carbon concentration (nmol C per L) | Estimated metabolite carbon concentration (nmol C per L) | Estimated metabolite carbon concentration per particulate carbon (nmol C per umol C) | Estimated metabolite carbon concentration (nmol C per L) |
Chromatography:
| Chromatography ID: | CH003098 |
| Chromatography Summary: | See attached summary. |
| Instrument Name: | Waters Acquity I-Class |
| Column Name: | SeQuant ZIC- pHILIC (150 x 2.1mm,5um) |
| Column Temperature: | 30 |
| Flow Gradient: | 100% B for 2 minutes, ramped to 64% A over 18 minutes, ramped up to 100% A over 1 minute, held at 100% A for 7 minutes, and equilibrated at 100% B for 22 minutes (total time is 50 minutes) |
| Flow Rate: | 0.15 mL/min |
| Solvent A: | 10mM ammonium carbonate;85% water/15% acetonitrile |
| Solvent B: | 10mM ammonium carbonate;85% acetonitrile/15% water |
| Chromatography Type: | HILIC |
| Chromatography ID: | CH003099 |
| Chromatography Summary: | See attached summary. |
| Instrument Name: | Waters Acquity I-Class |
| Column Name: | Waters ACQUITY UPLC CSH C18 (150 x 2.1mm,1.7um) |
| Column Temperature: | 65 |
| Flow Gradient: | Initial conditions were 90% A and 10% B. The column was ramped to 80% B over 33 minutes, ramped up to 90% B over 12 minutes, held at 90% B for 1 minute, and equilibrated to 90%A and 10% B for 6 minutes (total time is 52 minutes). |
| Flow Rate: | 0.45 mL/min |
| Solvent A: | 10mM ammonium formate; 60% acetonitrile/40% water; 0.1% formic acid |
| Solvent B: | 10mM ammonium formate; 90% isopropyl alcohol/10% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
| Chromatography ID: | CH003100 |
| Chromatography Summary: | See attached summary. |
| Instrument Name: | Waters Acquity I-Class |
| Column Name: | Waters ACQUITY UPLC HSS CN (100 x 2.1mm,1.8um) |
| Column Temperature: | 35 |
| Flow Gradient: | The column was held at 5% B for 2 minutes, ramped to 100% B over 18 minutes, held at 100% B for 2 minutes, and equilibrated at 5% B for 5 minutes (total run time is 25 minutes) |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 0.1% formic acid in water |
| Solvent B: | 0.1% formic acid in acetonitrile |
| Chromatography Type: | Reversed phase |
