Summary of Study ST002696

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001668. The data can be accessed directly via it's Project DOI: 10.21228/M83H7N This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002696
Study TitleDeficiency of the lipid flippase ATP10A causes diet-induced dyslipidemia in female mice
Study TypeMS Untargeted Lipidomics
Study SummaryGenetic association studies have linked ATP10A and closely related type IV P-type ATPases (P4-ATPases) to insulin resistance and vascular complications, such as atherosclerosis. In addition, prior studies of mice harboring large, overlapping chromosomal deletions implicated Atp10A in the development of diet-induced obesity and insulin resistance. Here, we generated gene-specific Atp10A knockout mice and show that Atp10A-/- mice fed a high-fat diet did not gain excess weight relative to wild-type littermates. However, Atp10A-/- mice displayed female-specific dyslipidemia characterized by elevated plasma triglycerides, free fatty acids and cholesterol, as well as altered VLDL and HDL properties. We also observed increased circulating levels of several sphingolipid species along with reduced levels of eicosanoids and bile acids. The Atp10A-/- mice also displayed hepatic insulin resistance without perturbations to whole-body glucose homeostasis. Thus, ATP10A has a sex-specific role in regulating plasma lipid composition and maintaining hepatic liver insulin sensitivity in mice.
Vanderbilt University
LaboratoryCenter for Innovative Technology
Last NameMay
First NameJody
Address2301 Vanderbilt Place, Nashville, TN, 37235, USA
Submit Date2023-04-20
Num Groups2
Total Subjects10
Raw Data AvailableYes
Raw Data File Type(s)d, mzML
Analysis Type DetailLC-MS
Release Date2023-05-24
Release Version1
Jody May Jody May application/zip

Select appropriate tab below to view additional metadata details:

Combined analysis:

Analysis ID AN004369 AN004370
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Agilent 6560 Agilent 6560
Column Agilent ZORBAX RRHD Extend-C18 (50 x 2.1mm,1.8um) Agilent ZORBAX RRHD Extend-C18 (50 x 2.1mm,1.8um)
MS instrument type QTOF QTOF
MS instrument name Agilent 6560 Ion Mobility Agilent 6560 Ion Mobility
Units Ion Abundances Ion Abundances


Chromatography ID:CH003275
Chromatography Summary:HPLC-IM-MS/MS on an Agilent 6560 mass spectrometer using a ZORBAX Extend-C18 RPLC column (Phase A: 0.1% formic acid and 10 mM NH4CHOO in water, Phase B: 0.1% formic acid and 10 mM NH4CHOO in 60:36:4 isopropanol:acetonitrile:water).
Instrument Name:Agilent 6560
Column Name:Agilent ZORBAX RRHD Extend-C18 (50 x 2.1mm,1.8um)
Column Temperature:40
Flow Gradient:70% B for 1 min, 70-86% B in 2.5 min, 86% B for 6.5 min, 86-100% B in 1 min, 100% B for 6 min, 100-70% B in 0.1 min, and 70% B for 1.9 min.
Flow Rate:300 ul/min
Solvent A:Water (10mM Amm. Formate) 0.1% formic acid
Solvent B:60:36:4 IPA:ACN:H2O (10mM Amm. Formate) 0.1% formic acid
Chromatography Type:Reversed phase