Summary of Study ST002947
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001832. The data can be accessed directly via it's Project DOI: 10.21228/M8X144 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002947 |
Study Title | Hepatic lipid profiles of wild type and Cgref1-depleted mice of C57BL/6 strain obtained by an untargeted LC-MS/MS analysis |
Study Summary | Among the range of experiments and observations recorded, we compared the lipid expression in the liver tissues between wild type and Cgref1-knockout mice of C57BL/6 strain using untargeted LC-MS/MS (n=3 per group). Particularly, the results revealed that Cgref1-knockout (KO) mice overall have lower levels of triglyceride and diglyceride species. Such finding provides supportive evidence that Cgref1 may promote de novo lipogenesis in the liver and increase the risk of fatty liver development. |
Institute | The University of Hong Kong |
Department | School of Biomedical Sciences |
Laboratory | L3-53 |
Last Name | Chan |
First Name | Pearl |
Address | 21 Sassoon Road, Pokfulam, Hong Kong |
pearl20@connect.hku.hk | |
Phone | +85239176812 |
Submit Date | 2023-10-26 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2024-10-28 |
Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
Analysis ID | AN004832 | AN004833 |
---|---|---|
Analysis type | MS | MS |
Chromatography type | Reversed phase | Reversed phase |
Chromatography system | Thermo Vanquish | Thermo Vanquish |
Column | Thermo Accucore C30 (150 x 2.1mm,2.6um) | Thermo Accucore C30 (150 x 2.1mm,2.6um) |
MS Type | ESI | ESI |
MS instrument type | Orbitrap | Orbitrap |
MS instrument name | Orbitrap Exploris 120 | Orbitrap Exploris 120 |
Ion Mode | POSITIVE | NEGATIVE |
Units | Peak Area | Peak Area |
Chromatography:
Chromatography ID: | CH003652 |
Instrument Name: | Thermo Vanquish |
Column Name: | Thermo Accucore C30 (150 x 2.1mm,2.6um) |
Column Temperature: | 45°C |
Flow Gradient: | The gradient started at 30% B and was increased to 43% B in 2 min, then increased to 55% B in 2.1 min, 65 % B in 12 min, 85% B in 18 min and 100 % B in 20 min, then held for 5min, and decreased linearly to 30% B for re-equilibration time at starting conditions. |
Flow Rate: | 0.26 mL min−1 |
Solvent A: | 10mM ammonium formate with 0.1% formic acid in acetonitrile and water, v/v 6:4 |
Solvent B: | 10mM ammonium formate with 0.1% formic acid in acetonitrile and IPA 1:9 |
Chromatography Type: | Reversed phase |