Summary of Study ST002947

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001832. The data can be accessed directly via it's Project DOI: 10.21228/M8X144 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002947
Study TitleHepatic lipid profiles of wild type and Cgref1-depleted mice of C57BL/6 strain obtained by an untargeted LC-MS/MS analysis
Study SummaryAmong the range of experiments and observations recorded, we compared the lipid expression in the liver tissues between wild type and Cgref1-knockout mice of C57BL/6 strain using untargeted LC-MS/MS (n=3 per group). Particularly, the results revealed that Cgref1-knockout (KO) mice overall have lower levels of triglyceride and diglyceride species. Such finding provides supportive evidence that Cgref1 may promote de novo lipogenesis in the liver and increase the risk of fatty liver development.
Institute
The University of Hong Kong
DepartmentSchool of Biomedical Sciences
LaboratoryL3-53
Last NameChan
First NamePearl
Address21 Sassoon Road, Pokfulam, Hong Kong
Emailpearl20@connect.hku.hk
Phone+85239176812
Submit Date2023-10-26
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2024-10-28
Release Version1
Pearl Chan Pearl Chan
https://dx.doi.org/10.21228/M8X144
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Combined analysis:

Analysis ID AN004832 AN004833
Analysis type MS MS
Chromatography type Reversed phase Reversed phase
Chromatography system Thermo Vanquish Thermo Vanquish
Column Thermo Accucore C30 (150 x 2.1mm,2.6um) Thermo Accucore C30 (150 x 2.1mm,2.6um)
MS Type ESI ESI
MS instrument type Orbitrap Orbitrap
MS instrument name Orbitrap Exploris 120 Orbitrap Exploris 120
Ion Mode POSITIVE NEGATIVE
Units Peak Area Peak Area

Chromatography:

Chromatography ID:CH003652
Instrument Name:Thermo Vanquish
Column Name:Thermo Accucore C30 (150 x 2.1mm,2.6um)
Column Temperature:45°C
Flow Gradient:The gradient started at 30% B and was increased to 43% B in 2 min, then increased to 55% B in 2.1 min, 65 % B in 12 min, 85% B in 18 min and 100 % B in 20 min, then held for 5min, and decreased linearly to 30% B for re-equilibration time at starting conditions.
Flow Rate:0.26 mL min−1
Solvent A:10mM ammonium formate with 0.1% formic acid in acetonitrile and water, v/v 6:4
Solvent B:10mM ammonium formate with 0.1% formic acid in acetonitrile and IPA 1:9
Chromatography Type:Reversed phase
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