Summary of Study ST003238
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002013. The data can be accessed directly via it's Project DOI: 10.21228/M8DJ7S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003238 |
| Study Title | Lipidomic analysis of serum from Gclc WT and whole-body Gclc KO mice. |
| Study Summary | We found decreased triglycerides in the serum of whole-body deletion of Gclc mice compared to wildtype mice. |
| Institute | University of Rochester Medical Center |
| Last Name | Harris |
| First Name | Isaac |
| Address | 601 Elmwood Ave, Rochester, New York, 14642-0001, USA |
| isaac_harris@urmc.rochester.edu | |
| Phone | 8572348624 |
| Submit Date | 2024-05-29 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-06-07 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005303 | AN005304 |
|---|---|---|
| Chromatography ID | CH004012 | CH004012 |
| MS ID | MS005033 | MS005034 |
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Brownlee SPP C18 (75 x 2.1mm, 2.7um) | Brownlee SPP C18 (75 x 2.1mm, 2.7um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Normalized to the median value of total lipid signals | Normalized to the median value of total lipid signals |
Chromatography:
| Chromatography ID: | CH004012 |
| Chromatography Summary: | Brownlee SPP C18 column (2.1 mm × 75 mm, 2.7 μm particle size); Solvent A is 100% H2O containing 0.1% formic acid and 1% of 1 M NH4OAc; Solvent B is 1:1 acetonitrile:isopropanol containing 0.1% formic acid and 1% of 1 M NH4OAc |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Brownlee SPP C18 (75 x 2.1mm, 2.7um) |
| Column Temperature: | 50 |
| Flow Gradient: | 0–2 min 35% B, 2–8 min from 35 to 80% B, 8–22 min from 80 to 99% B, 22–36 min 99% B, and 36.1–40 min from 99 to 35% B. |
| Flow Rate: | 0.400 mL/min |
| Solvent A: | 100% water; 0.1% formic acid; 10mM ammonium acetate |
| Solvent B: | 50% acetonitrile/50% isopropanol; 0.1% formic acid; 10mM ammonium acetate |
| Chromatography Type: | Reversed phase |
