Summary of Study ST003245

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR002015. The data can be accessed directly via it's Project DOI: 10.21228/M85238 This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003245
Study TitleExploration of Zeb1-dependent changes in the lipidome of MDA-MB-231 cells
Study SummaryHuman breast cancer MDA-MB-231 wildtype (WT) cells and the stably transduced MDA-MB-231 shZeb1 (stable Zeb1 knockdown) and shCtrl cell lines (control cell line for the stable Zeb1 knockdown) (Spaderna et al. 2008, DOI: 10.1158/0008-5472.CAN-07-5682) were analyzed for their phospholipid profile by UPLC-MS/MS. Please note that one sample set was measured three times with the same sample-ID, but with different methods (PE, PC, PI), therefore each sub-class has their own raw-data file marked by their corresponding abbreviation (PE, PC, PI; e.g. "210514_MDA_ZEB1_PE_dil_1_10_std_1ul_JZ_PE_MRM_002.wiff", "210514_MDA_ZEB1_PC_dil_1_10_std_1ul_JZ_PC_MRM_002.wiff" or "210514_MDA_ZEB1_PC_dil_1_10_std_1ul_JZ_PC_MRM_002.wiff").
University of Innsbruck
DepartmentMichael Popp Institute
Last NameKoeberle
First NameAndreas
AddressMitterweg 24, Innsbruck, Tyrol, 6020, Austria
Phone+43 512 507 57903
Submit Date2024-05-27
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2024-07-05
Release Version1
Andreas Koeberle Andreas Koeberle application/zip

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Combined analysis:

Analysis ID AN005315
Analysis type MS
Chromatography type Reversed phase
Chromatography system Waters Acquity H-Class
Column Waters ACQUITY UPLC BEH C8 (100 x 2.1mm,1.7um)
MS instrument type QTRAP
MS instrument name ABI Sciex 6500+
Units relative intensities


Chromatography ID:CH004019
Chromatography Summary:Chromatographic separation of phospholipids was carried out on an Acquity BEH C8 column (1.7 μm, 2.1×100 mm, Waters, Milford, MA) using an Acquity UHPLC.
Instrument Name:Waters Acquity H-Class
Column Name:Waters ACQUITY UPLC BEH C8 (100 x 2.1mm,1.7um)
Column Temperature:45
Flow Gradient:The gradient was ramped from 75 to 85% B over 5 min and further increased to 100% B within 2 min, followed by isocratic elution for another 2 min
Flow Rate:0.75 mL/min
Solvent A:90% Water, 10% acetonitrile; 2 mM ammonium acetate
Solvent B:95% Acetonitrile, 5% Water; 2 mM ammonium acetate
Chromatography Type:Reversed phase