Summary of Study ST003398
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002104. The data can be accessed directly via it's Project DOI: 10.21228/M8NJ9Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003398 |
| Study Title | Specific activation of the integrated stress response (ISR) uncovers regulation of lipid droplet biogenesis |
| Study Type | Biology |
| Study Summary | U2OS cells were treated with Dimerizer-PERK for 0h,1h,2h,4h,8h and 24h. Lipidomics analysis using LC-MS was performed on these samples to understand the regulation of cellular lipidome upon ISR activation |
| Institute | Calico Life Sciences |
| Department | Department of Mass Spectrometry-Technology Lab |
| Laboratory | Metabolomics Lab |
| Last Name | Vu |
| First Name | Ngoc |
| Address | 1130 Veterans BLVD, South San Francisco, CA 94080 |
| ngoc@calicolabs.com | |
| Phone | 650-420-5430 |
| Submit Date | 2024-08-08 |
| Num Groups | 6 |
| Total Subjects | 18 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-09-03 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005577 | AN005578 |
|---|---|---|
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Thermo Scientific Accucore C30 (250 × 2.1mm, 2.6um, 150 Å) | Thermo Scientific Accucore C30 (250 × 2.1mm, 2.6um, 150 Å) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive Plus Orbitrap | Thermo Q Exactive Plus Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Log2(Top Peak Area) | Log2(Top Peak Area) |
Chromatography:
| Chromatography ID: | CH004238 |
| Chromatography Summary: | RPLC with a Thermo Accucore C30 for lipidomics analysis |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Thermo Scientific Accucore C30 (250 × 2.1mm, 2.6um, 150 Å) |
| Column Pressure: | 900 bar |
| Column Temperature: | 35 |
| Flow Gradient: | The gradient was t(min) = −7, 30% B, t = 0, 30% B, t = 7, 43% B, t = 12, 65% B, t = 30, 70% B, t = 31, 88% B, t = 51, 95% B, t = 53, 100% B, t = 55, 100% B, t = 55.1, 30% B, t = 60, 30% B. |
| Flow Rate: | 200uL/min |
| Injection Temperature: | 4C |
| Internal Standard: | Splash Lipidomix (Avanti Polar Lipids) |
| Solvent A: | 60% acetonitrile/40% water; 20mM ammonium formate; 0.25μM medronic acid |
| Solvent B: | 90% isopropanol/10% acetonitrile; 20mM ammonium formate; 0.25μM medronic acid |
| Chromatography Type: | Reversed phase |
