Summary of Study ST003399
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002105. The data can be accessed directly via it's Project DOI: 10.21228/M8HV52 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003399 |
| Study Title | Chemical Biology Meets Metabolomics: The Response of Barley Seedlings to 3,5-Dichloroanthranilic Acid, a Resistance Inducer |
| Study Type | Plant metabolomics |
| Study Summary | Advances in combinatorial synthesis and high-throughput screening methods have led to renewed interest in synthetic plant immune activators as well as priming agents. 3,5-Dichloroanthranilic acid (3,5-DCAA) is a derivative of anthranilic acid that have shown potency in activating defence mechanisms in Arabidopsis and barley plants. Chemical biology which is the interface of chemistry and biology can make use of metabolomics approaches and tools to better understand molecular mechanisms operating in complex biological systems. Aim: Here we report on the untargeted metabolomics profiling of barley seedlings treated with 3,5-DCAA to gain deeper insights into the mechanism of action of this resistance inducer. Methodology: Hydro-methanolic extracts from different time periods (12, 24 and 36 h post-treatment) were analysed on ultra-high performance liquid chromatography hyphenated with a high-resolution mass spectrometer. Both unsupervised and supervised chemometric methods were used to reveal hidden patterns and highlight metabolite variables associated with the treatment. Results: Based on the metabolites identified, both the phenylpropanoid and octadecanoid pathways appear to be main route activated by 3,5-DCAA. Different classes of responsive metabolites were annotated with favonoids, more especially flavones, the most dominant. Given the limited understanding of this inducer, this study offers a metabolomics analysis of the response triggered by its foliar application in barley. This additional insight could help make informed decision for the development of more effective strategies for crop protection and improvement, ultimately contributing to agricultural sustainability and resilience. |
| Institute | University of Johannesburg |
| Department | Biochemistry |
| Laboratory | Plant metabolomics |
| Last Name | Claude Yasmine Hamany Djande |
| First Name | Claude Yasmine |
| Address | 81A Fourth Avenue Westdene |
| claudehamany@gmail.com | |
| Phone | 0814415123 |
| Submit Date | 2024-07-05 |
| Num Groups | 4 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Waters) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-09-03 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005579 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Waters Acquity |
| Column | Waters HSS T3 C18 (150 x 2.1mm x 1.8um) |
| MS Type | ESI |
| MS instrument type | QTOF |
| MS instrument name | Waters SYNAPT G1 QTOF |
| Ion Mode | NEGATIVE |
| Units | m/z_rt |
Chromatography:
| Chromatography ID: | CH004239 |
| Chromatography Summary: | The analysis of the aqueous methanol extracts was performed with a Waters Acquity UHPLC coupled to a Waters SYNAPT G1 QTOF (quadrupole time-of-flight) high definition mass spectrometer system (Waters Corporation, Milford, MA, USA). The HSS T3 C18 column (150 mm x 2.1 mm x 1.8 µm, Waters Corporation) was thermostatted at 60 °C and used for the reverse phase chromatographic separation of extracts. The mobile phase consisted of mass spectrometry grade water and acetonitrile (Romil, SpS, Cambridge, UK) and formic acid (Sigma-Aldrich, Merck, Johannesburg, South Africa). Eluents A (water), and B (acetonitrile), both containing 0.1% formic acid were used for the concave gradient elution running at a flow rate of 0.4 mL min−1. The elution commenced with 5% B for the first min, and gradually increased to 95% B over 24 min. The chromatographic conditions were then adjusted to 10% A and 90% B, for 10 s, followed by 5% A and 95% B for 1 min 50 s before restoration to the initial conditions for column equilibration for 2 min. The injection volume was 2 µL and the total run time was 30 min. To account for analytical variability and to prevent measurement bias, each sample was analysed in triplicate. The sample order was randomised and blanks consisting of 50% methanol were injected to monitor the background noise, possible sample carry-over and solvent contamination. The stability of the LC-MS system was monitored by inserting quality control (QC) samples in the batches. Data acquisition involved three independent biological replicates, with each replicate analysed in triplicate, resulting in a total sample size of n = 9. |
| Instrument Name: | Waters Acquity |
| Column Name: | Waters HSS T3 C18 (150 x 2.1mm x 1.8um) |
| Column Temperature: | 60 |
| Flow Gradient: | The elution commenced with 5% B for the first min, and gradually increased to 95% B over 24 min. The chromatographic conditions were then adjusted to 10% A and 90% B, for 10 s, followed by 5% A and 95% B for 1 min 50 s before restoration to the initial conditions for column equilibration for 2 min. The injection volume was 2 µL and the total run time was 30 min |
| Flow Rate: | 0.4 mL/min |
| Solvent A: | 100% water; 0.1% formic acid |
| Solvent B: | 100% acetonitrile; 0.1% formic acid |
| Chromatography Type: | Reversed phase |
