Summary of Study ST003552
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002188. The data can be accessed directly via it's Project DOI: 10.21228/M8SV5J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003552 |
| Study Title | Investigation of age-dependent changes in the gut of honeybee workers using targeted metabolomics for SCFA determination |
| Study Summary | To determine changes in the metabolic activity of the gut microbiota, we measured the concentrations of seven short-chain fatty acids (SCFA) and lactate. All SCFA showed low concentrations in newly emerged workers. We found that acetate, propionate and butyrate are the main SCFA in the honeybee gut and peak in 5-day old and in-hive workers. Lactate showed an inverse trend with highest concentrations in 1-day old workers and a significant decrease on all subsequent days (Kruskal Wallis test, p <0.05). |
| Institute | Helmholtz Centre for Environmental Research |
| Department | Molecular Systems Biology |
| Last Name | Engelmann |
| First Name | Beatrice |
| Address | Permoserstraße 15, Leipzipg, Saxony, 03418, Germany |
| beatrice.engelmann@ufz.de | |
| Phone | 004934160251099 |
| Submit Date | 2024-10-21 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-11-27 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN005839 |
|---|---|
| Analysis type | MS |
| Chromatography type | Reversed phase |
| Chromatography system | Thermo Dionex Ultimate 3000 RS |
| Column | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| MS Type | ESI |
| MS instrument type | Triple quadrupole |
| MS instrument name | ABI Sciex 5500 QTrap |
| Ion Mode | NEGATIVE |
| Units | µMol |
Chromatography:
| Chromatography ID: | CH004435 |
| Instrument Name: | Thermo Dionex Ultimate 3000 RS |
| Column Name: | Waters ACQUITY UPLC BEH C18 (100 x 2.1mm,1.7um) |
| Column Temperature: | 40 |
| Flow Gradient: | 0-2min: 15% B, 2-17min: 15-50% B, 17-17.1min: 50-100% B, 17.1-18min: 100% B, 18-18.1min: 100-15% B, 18.1-21min: 15% B |
| Flow Rate: | 0.35 mL/min |
| Solvent A: | 100% water; 0.01% formic acid |
| Solvent B: | 100% acetonitrile; 0.01% formic acid |
| Chromatography Type: | Reversed phase |
