Summary of Study ST004264
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002691. The data can be accessed directly via it's Project DOI: 10.21228/M8T837 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST004264 |
| Study Title | Dynamic metabolic and molecular changes during seasonal shrinking in Sorex araneus |
| Study Type | MS quantitative analysis |
| Study Summary | To meet the challenge of wintering in place many high-latitude small mammals reduce energy demands through hibernation. In contrast, short-lived Eurasian common shrews, Sorex araneus, remain active and shrink, including energy-intensive organs in winter, regrowing in spring in an evolved strategy called Dehnel’s phenomenon. How this size change is linked to metabolic and regulatory changes to sustain their high metabolism is unknown. We analyzed metabolic, proteomic, and gene expression profiles spanning the entirety of Dehnel’s seasonal cycle in wild shrews. We show regulatory changes to oxidative phosphorylation and increased fatty acid metabolism during autumn-to-winter shrinkage, as previously found in hibernating species. But in shrews we also found upregulated winter expression of genes involved in gluconeogenesis: the biosynthesis of glucose from non-carbohydrate substrates. Co-expression models revealed changes in size and metabolic gene expression interconnect via FOXO signaling, whose overexpression reduces size and extends lifespan in many model organisms. We propose that while shifts in gluconeogenesis meet the challenge posed by high metabolic rate and active winter lifestyle, FOXO signaling is central to Dehnel’s phenomenon, with spring downregulation limiting lifespan in these shrews. |
| Institute | Aalborg University |
| Department | Health Science and Technology |
| Laboratory | Molecular Pharmacology |
| Last Name | Zeng |
| First Name | Yuanyuan |
| Address | Thulevej 34 Aalborg SØ, Aalborg, Aalborg, 9210, Denmark |
| yuanyzeng@outlook.com | |
| Phone | +4555204123 |
| Submit Date | 2025-09-16 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-11-03 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Combined analysis:
| Analysis ID | AN007096 | AN007097 |
|---|---|---|
| Chromatography ID | CH005390 | CH005390 |
| MS ID | MS006793 | MS006794 |
| Analysis type | MS | MS |
| Chromatography type | Reversed phase | Reversed phase |
| Chromatography system | Thermo Vanquish | Thermo Vanquish |
| Column | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| MS Type | ESI | ESI |
| MS instrument type | Orbitrap | Orbitrap |
| MS instrument name | Thermo Q Exactive HF hybrid Orbitrap | Thermo Q Exactive HF hybrid Orbitrap |
| Ion Mode | POSITIVE | NEGATIVE |
| Units | Peak area | Peak area |
Chromatography:
| Chromatography ID: | CH005390 |
| Chromatography Summary: | The analysis was carried out using a UPLC system (Vanquish, Thermo Fisher Scientific) coupled with a high-resolution quadrupole-orbitrap mass spectrometer (Q Exactive™ HF Hybrid Quadrupole-Orbitrap, Thermo Fisher Scientific). An electrospray ionization interface was used as ionization source. Analysis was performed in negative and positive ionization mode. A QC sample was analysed in MS/MS mode for identification of compounds. The UPLC was performed using a slightly modified version of the protocol described by Catalin et al. (UPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes, Water Application note 2011, 720004042en). |
| Methods Filename: | Waters_Water_Soluble_Vitamin-Bs.pdf |
| Instrument Name: | Thermo Vanquish |
| Column Name: | Waters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um) |
| Column Temperature: | 30 °C |
| Flow Gradient: | 0.0 min 0% B 2.0 min 0% B 12.0 min 35% B 13.0 min 90% B 14.0 min 90% B 15.0 min 0% B |
| Flow Rate: | 300 μL/min |
| Solvent A: | 100% water; 10 mM ammonium formate; 0.1% formic acid (pH 3.1) |
| Solvent B: | 100% methanol; 10 mM ammonium formate, 0.1% formic acid |
| Chromatography Type: | Reversed phase |
