Summary of Study ST000049

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000024. The data can be accessed directly via it's Project DOI: 10.21228/M85P4V This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Download binned data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST000049
Study TitleMetabolomics Analysis of Thermally Challenged Mayfly Larvae (NMR analysis)
Study TypeMetabolomic analysis of mayflies
Study SummaryThe purpose of this study was to examine the metabolic profiles of mayfly (Centroptilum triangulifer) larvae subjected to thermal challenge. This species is unusual in terms of its ease of culture, and its suitability as a laboratory test organism. Our purpose here was to examine how an environmentally realistic thermal challenge affects the physiology of this organism. In this study, we obtained several types of insect species and we were able to show that NMR Metabolomics could be used to distinguish among the different types of larvae.
Institute
University of North Carolina
DepartmentDiscovery Science Technology
LaboratorySumner Lab
Last NameSumner
First NameSusan
AddressEastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Emailsusan_sumner @unc.edu
Phone704-250-5066
Submit Date2014-02-28
Num Groups7
Raw Data AvailableYes
Raw Data File Type(s)fid
Uploaded File Size7.6 M
Analysis Type DetailNMR
Release Date2015-05-01
Release Version1
Susan Sumner Susan Sumner
https://dx.doi.org/10.21228/M85P4V
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Collection:

Collection ID:CO000050
Collection Summary:whole insects were collected
Collection Protocol Comments:Original culture material was obtained from the Stroud Water Research Center (Avondale, PA). Larvae were reared at 22°C and fed a periphytic diet. When larvae reached a suitable size, they were separated from their food source for 12 hours to evacuate gut contents. Larvae were then divided into two treatment groups – a control group maintained at the culturing temperature of 22°C, and a thermal challenge group. The thermal challenge group was subjected to a temperature increase at a rate of 1°C per hour. This rate of temperature change is commonly observed in temperate streams. When the treatment temperature reached 30°C, larvae from both control and treatment groups were flash frozen in liquid nitrogen in groups of 12-13 larvae. Each grouping of 12-13 larvae comprised a composite replicate for the metabolic profiling analysis. A total of 3 replicates each for the control and thermal treatments were used in this study. Some larvae had developed dark wing pads during the experiment. This is a signal that the larvae were very close to emerging from the aquatic larval phase to a winged sub-adult phase. Enough of these larvae were present in the control treatment to be removed from the control cohort and considered as an independent sample.
Sample Type:whole insect
Volumeoramount Collected:50 mg
Storage Conditions:-80C
  logo