Summary of study ST000121

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000108. The data can be accessed directly via it's Project DOI: 10.21228/M8GW2Z This work is supported by NIH grant, U2C- DK119886.

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Study IDST000121
Study TitleImpact of anesthesia and euthanasia on metabolomics of mammalian tissues: studies in a C57BL/6J mouse model
Study TypeAnesthesia effect study
Study SummaryWe examined the effect of several commonly-used methods of anesthesia and euthanasia for collection of skeletal muscle, liver, heart, adipose and serum of C57BL/6J mice. The data revealed tissue-specific impacts of different anesthesia and euthanasia strategies. Based on these findings, we present a more optimal collection strategy mammalian tissues and recommend that rodent tissues intended for metabolomics studies be collected under anesthesia rather than post-euthanasia.
Institute
University of Michigan
DepartmentInternal Medicine
LaboratoryEvans Lab / Burant Lab
Last NameEvans
First NameCharles
Address6321 Brehm Tower, 1000 Wall Street, Ann Arbor MI 48105
Emailchevans@umich.edu
Phone734-232-8177
Submit Date2014-11-21
Num Groups6
Total Subjects49
Raw Data AvailableNo
Analysis Type DetailLC-MS
Release Date2015-02-01
Release Version1
Charles Evans Charles Evans
https://dx.doi.org/10.21228/M8GW2Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO000124
Collection Summary:4 tissues plus blood serum were collected and immediately following euthanasia or anesthesia induction, frozen in liquid nitrogen and stored at -80C until analysis
Sample Type:Skeletal muscle (gastrocnemius), Liver, Heart, Epididymal white adipose (fat), blood serum
Collection Method:Surgical dissection
Collection Time:As soon as possible following anesthesia/euthanasia (all tissues collected within 3 minutes)
Storage Conditions:-80 C
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