Summary of Study ST000121
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000108. The data can be accessed directly via it's Project DOI: 10.21228/M8GW2Z This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000121 |
Study Title | Impact of anesthesia and euthanasia on metabolomics of mammalian tissues: studies in a C57BL/6J mouse model |
Study Type | Anesthesia effect study |
Study Summary | We examined the effect of several commonly-used methods of anesthesia and euthanasia for collection of skeletal muscle, liver, heart, adipose and serum of C57BL/6J mice. The data revealed tissue-specific impacts of different anesthesia and euthanasia strategies. Based on these findings, we present a more optimal collection strategy mammalian tissues and recommend that rodent tissues intended for metabolomics studies be collected under anesthesia rather than post-euthanasia. |
Institute | University of Michigan |
Department | Internal Medicine |
Laboratory | Evans Lab / Burant Lab |
Last Name | Evans |
First Name | Charles |
Address | 6321 Brehm Tower, 1000 Wall Street, Ann Arbor MI 48105 |
chevans@umich.edu | |
Phone | 734-232-8177 |
Submit Date | 2014-11-21 |
Num Groups | 6 |
Total Subjects | 49 |
Raw Data Available | No |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2015-02-01 |
Release Version | 1 |
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Collection:
Collection ID: | CO000124 |
Collection Summary: | 4 tissues plus blood serum were collected and immediately following euthanasia or anesthesia induction, frozen in liquid nitrogen and stored at -80C until analysis |
Sample Type: | Skeletal muscle (gastrocnemius), Liver, Heart, Epididymal white adipose (fat), blood serum |
Collection Method: | Surgical dissection |
Collection Time: | As soon as possible following anesthesia/euthanasia (all tissues collected within 3 minutes) |
Storage Conditions: | -80 C |