Summary of Study ST000140

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000125. The data can be accessed directly via it's Project DOI: 10.21228/M8DW2B This work is supported by NIH grant, U2C- DK119886.

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Study IDST000140
Study TitleCell Rinsing Solution Comparison
Study TypeCell Rinsing Solution Comparison
Study SummaryAliquots of Jurkat T-lymphocyte cells were washed with 5 different rinsing solutions (0.3% amm. formate, 0.3% amm. acetate, 0.9% NaCl, 1 M PBS, 100 mM PBS) and ran in triplicate to monitor the effect of ion suppression on the electrospray ionization signal.
Institute
University of Florida
DepartmentDept. of Chemistry/SECIM
LaboratoryBiomedical Mass Spectrometry Lab
Last NameUlmer
First NameCandice
AddressP.O. Box 117200, Gainesville, FL 32611
Emailczulmer@chem.ufl.edu
Phone(352) 392-0515
Submit Date2015-03-02
Num Groups5
Total Subjects15
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Uploaded File Size3.1 G
Analysis Type DetailLC-MS
Release Date2016-03-03
Release Version1
Candice Ulmer Candice Ulmer
https://dx.doi.org/10.21228/M8DW2B
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO000144
Sample Type:Jurkat cells, Clone E6-1
Collection Method:Centrifugation
Collection Frequency:1200 rpm
Collection Duration:5 min.
Storage Conditions:-80°C
Tissue Cell Quantity Taken:1e6 cells
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