Summary of Study ST000247

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000199. The data can be accessed directly via it's Project DOI: 10.21228/M8P304 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000247
Study TitleDetermining the metabolic profile of wildtype and nos mutant Staphylococcus aureus grown in media lacking glucose using targeted LC/MS
Study TypeSingle time point
Study SummaryWhole cells from wildtype, nos mutant, SrrAB mutant, SrrAB nos double mutant, and complement strains will be isolated for targeted metabolite analysis. In parallel, supernatants (extracellular metabolites) will also be analyzed for their metabolic profile.
Institute
University of Florida
DepartmentSECIM
Last NameRice
First NameKelly
Emailkcrice@ufl.edu
Submit Date2015-02-17
Num Groups7
Total Subjects21
Raw Data File Type(s)raw(Thermo), d
Analysis Type DetailLC-MS
Release Date2019-01-22
Release Version1
Kelly Rice Kelly Rice
https://dx.doi.org/10.21228/M8P304
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO000258
Collection Summary:The wildtype S. aureus, nos mutant, SrrAB mutant, nos SrrAB double mutant, and complement strains will be grown in 40 ml trytic soy broth lacking dextrose for 4 hours. At 4 hours growth, all 40 ml was isolated, centrifuged at 4500 rpms and 4C for 10 minutes and then placed on ice. 1 ml of supernatant was removed in duplicate and saved as supernatant. The rest of the supernatant was discarded and pellets were resuspended in 2 ml PBS, centrifuged at 4500 rpms for 3 minutes (4C). Supernatant was discarded and samples were again resuspended in 2 ml PBS. These samples were split into 2 separate 1 ml aliquots (for metabolite and protein determination) and frozen with liquid nitrogen. Separate media only samples of Tryptic Soy Broth without dextrose were isolated for each day (3 total) Samples were stored at -80C. A total of 39 samples (18 whole cell, 18 supernatant, and 3 media) were isolated.
Collection Protocol Filename:Rice_Collections_targeted_20150807.txt
Sample Type:Bacterial cells
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