Summary of Study ST000964
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000662. The data can be accessed directly via it's Project DOI: 10.21228/M83Q2T This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000964 |
Study Title | U13C Glucose Tracing of Young and Old Pancreatic Islets |
Study Summary | Pancreatic islets from young and old mice were traced with either 2.8mM or 16.8mM U13C glucose to determine the effect of age upon glucose metabolism in basal and stimulated states. |
Institute | University of California, San Diego |
Last Name | Wortham |
First Name | Matthew |
Address | 2880 Torrey Pines Scenic Drive, Sanford Consortium for Regenerative Medicine, Room 3102 |
mwortham@ucsd.edu | |
Phone | 8582460588 |
Submit Date | 2018-04-27 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | GC-MS |
Release Date | 2019-04-27 |
Release Version | 1 |
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Collection:
Collection ID: | CO000997 |
Collection Summary: | Pancreata were digested using Liberase TL, and islets were purified using a Histopaque gradient followed by hand-picking under a dissecting microscope. Islets were allowed to recover overnight in RPMI 1640 media supplemented with 8 mM glucose, 10% FBS, 2 mM L-glutamine, 100 U/mL Pen/Strep, 1 mM sodium pyruvate, 10 mM HEPES, and 0.25 mg/mL amphoterecin B. |
Collection Protocol Filename: | Methods.pdf |
Sample Type: | Pancreatic Islets |