Summary of Study ST001023
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000684. The data can be accessed directly via it's Project DOI: 10.21228/M8868G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001023 |
| Study Title | H3K27M cells and glutamine metabolomics 1 million cell test (part-I) |
| Study Summary | Testing TCA concentrations of Diffuse Intrinsic Pontine Gliomas (DIPG) cellines with H3K27M mutations. One million cells are tested with a TCA concentrations panel. We are a high volume center for treating malignant gliomas, which gives us an advantage in obtaining tissue for these relatively rare tumors. We have developed several DIPG patient derived cell lines and xenografts that bear all the key molecular features of this disease including the H3K27M mutation and global H3K27 hypomethylation. These cells are low in passage and we think these lines more closely resemble the patients tumor pathology then established cell lines that have been in culture/mice for numerous years. |
| Institute | Mayo Clinic |
| Last Name | Daniels |
| First Name | David |
| Address | 200 First Street SW Rochester, MN 55905 |
| daniels.david@mayo.edu | |
| Phone | 507-284-2511 |
| Submit Date | 2018-07-18 |
| Analysis Type Detail | GC-MS |
| Release Date | 2020-07-15 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO001056 |
| Collection Summary: | DIPG XIII and DIPG XVII cell lines are collected in this experiment. Susupension cells are harvested using centrifugation at 1200 rpm for 5 min. 1 mL of the supernatant media werr collected in an eppendorf tube and snapped frozen. The cell pellets were broken up into single cell suspension and counted. 1 million cells were taken from the stock and washed 1 x with PBS using table top centrifuge with 10 sec quick spin. The resulting cell pellet was snap frozen. Both the frozen media and cell pellet are stored in -80 C prior transfer. |
| Sample Type: | Glioma cells |
