Summary of Study ST001032

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000690. The data can be accessed directly via it's Project DOI: 10.21228/M8GQ3M This work is supported by NIH grant, U2C- DK119886.

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Study IDST001032
Study TitleSingle-cell Profiling of Cationic and Anionic Metabolites in Live Frog (Xenopus) Embryos using Microprobe Capillary Electrophoresis Mass Spectrometry
Study TypeMetabolic profiling of single cells
Study SummaryThe goal of this study was to enable the analysis of anionic and cationic metabolites from the same identified single cell in Xenopus laevis embryos. A 10 nL portion of identified animal-ventral (V1) cells was aspirated from 8-cell embryos, and metabolites were extracted from the aspirate, before characterization of cationic and anionic compounds using a custom-built capillary electrophoresis (CE) electrospray ionization (ESI) mass spectrometry platform. A total of ~250 cationic molecular features and ~150 anionic molecular features were detected, including 76 metabolites that were identified in this study. Pathway analysis of the identified metabolites highlighted arginine-proline metabolism of significance.
Institute
University of Maryland
DepartmentDepartment of Chemistry & Biochemistry
LaboratoryNemes Laboratory
Last NameNemes
First NamePeter
Address0107 Chemistry Building 8051 Regents Drive
Emailnemes@umd.edu
Phone3014050373
Submit Date2018-08-08
Num Groups4 biological replicates (each different cell from a different embryo) + 1-to-2 technical replicates (same extract analyzed multiple times)
Total Subjects4 different V1 cells were analyzed, each from a different embryo
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2019-09-23
Release Version1
Peter Nemes Peter Nemes
https://dx.doi.org/10.21228/M8GQ3M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO001065
Collection Summary:Cells were identified based on morphology, pigmentation, and location in the embryo in comparison to established cell-fate maps for Xenopus laevis embryos. A portion of the identified V1 cell was microaspirated using a fabricated microcapillary.
Collection Protocol ID:Portero 2018 Metabolomics Workbench Protocols FINAL 2018-08-08
Collection Protocol Filename:nemes_20180725_143242_PR_SP_Liu_2018_Metabolomics_Workbench_Protocol.pdf
Sample Type:embryonic cell
Collection Method:Microaspiration of cell content
Collection Frequency:1 collection per cell
Collection Duration:5 s for aspiration
Volumeoramount Collected:Ca. 10 nL per aspiration
Storage Conditions:-80℃
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