Summary of Study ST001032
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000690. The data can be accessed directly via it's Project DOI: 10.21228/M8GQ3M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001032 |
Study Title | Single-cell Profiling of Cationic and Anionic Metabolites in Live Frog (Xenopus) Embryos using Microprobe Capillary Electrophoresis Mass Spectrometry |
Study Type | Metabolic profiling of single cells |
Study Summary | The goal of this study was to enable the analysis of anionic and cationic metabolites from the same identified single cell in Xenopus laevis embryos. A 10 nL portion of identified animal-ventral (V1) cells was aspirated from 8-cell embryos, and metabolites were extracted from the aspirate, before characterization of cationic and anionic compounds using a custom-built capillary electrophoresis (CE) electrospray ionization (ESI) mass spectrometry platform. A total of ~250 cationic molecular features and ~150 anionic molecular features were detected, including 76 metabolites that were identified in this study. Pathway analysis of the identified metabolites highlighted arginine-proline metabolism of significance. |
Institute | University of Maryland |
Department | Department of Chemistry & Biochemistry |
Laboratory | Nemes Laboratory |
Last Name | Nemes |
First Name | Peter |
Address | 0107 Chemistry Building 8051 Regents Drive |
nemes@umd.edu | |
Phone | 3014050373 |
Submit Date | 2018-08-08 |
Num Groups | 4 biological replicates (each different cell from a different embryo) + 1-to-2 technical replicates (same extract analyzed multiple times) |
Total Subjects | 4 different V1 cells were analyzed, each from a different embryo |
Raw Data Available | Yes |
Raw Data File Type(s) | mzML |
Analysis Type Detail | LC-MS |
Release Date | 2019-09-23 |
Release Version | 1 |
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Collection:
Collection ID: | CO001065 |
Collection Summary: | Cells were identified based on morphology, pigmentation, and location in the embryo in comparison to established cell-fate maps for Xenopus laevis embryos. A portion of the identified V1 cell was microaspirated using a fabricated microcapillary. |
Collection Protocol ID: | Portero 2018 Metabolomics Workbench Protocols FINAL 2018-08-08 |
Collection Protocol Filename: | nemes_20180725_143242_PR_SP_Liu_2018_Metabolomics_Workbench_Protocol.pdf |
Sample Type: | embryonic cell |
Collection Method: | Microaspiration of cell content |
Collection Frequency: | 1 collection per cell |
Collection Duration: | 5 s for aspiration |
Volumeoramount Collected: | Ca. 10 nL per aspiration |
Storage Conditions: | -80℃ |