Summary of study ST001124

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000732. The data can be accessed directly via it's Project DOI: 10.21228/M82D79 This work is supported by NIH grant, U2C- DK119886.

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Study IDST001124
Study TitleEarly Mechanistic Events Induced by Secondhand Smoke Prevalent Low Molecular Weight Polycyclic Aromatic Hydrocarbons in Mouse Lung Epithelial Cells
Study SummaryWe evaluated lung epithelial cells exposed to low molecular weight polycyclic aromatic hydrocarbons and what lipid metabolites were produced following early exposure, prior to metabolism. For the targeted study (AKB study 2, we used 40uM dose of the 2PAHs (1methylanthracene and fluoranthene; 1:1 ratio)and assessed 2,4,8,and 12 hrs of treatment with the PAHs. We also examined a 24 h time point in another study at a lower dose (15 uM LMW PAH mixture; 1:1 ratio of 1-methanthrancene and fluoranthene).
Institute
University of Colorado, Denver
DepartmentAnschutz Medical Campus
Last NameBauer
First NameAlison
Address12850 E. Montview Dr. Rm 3125, Aurora, CO 80045
Emailalison.bauer@ucdenver.edu
Phone303-724-6297
Submit Date2018-11-06
Raw Data AvailableYes
Raw Data File Type(s).cd,.cg, .xml, .bin, .stg
Analysis Type DetailLC-MS
Release Date2019-02-27
Release Version1
Alison Bauer Alison Bauer
https://dx.doi.org/10.21228/M82D79
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Collection:

Collection ID:CO001179
Collection Summary:Immediately following treatment, cell monolayers were washed 3 times with cold PBS, and cells were scraped and fixed in 500 μL 70% MeOH. Samples were stored immediately at -80°C until pretreatment for solid phase extraction (SPE), as follows.
Collection Protocol Filename:AKB_Targeted-protocol.docx
Collection Protocol Comments:For AKB study 2 protocol, the collection, treatment, and sample prep are all in the same file.
Sample Type:Epithelial cells
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