Summary of Study ST001294
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000876. The data can be accessed directly via it's Project DOI: 10.21228/M8FX1M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001294 |
| Study Title | Estimating Platelet Mitochondrial Function in Patients with Sepsis - Platelet NMRs (part-I) |
| Study Type | single timepoint |
| Study Summary | Relationships between platelet mitochondrial oxygen consumption rates (mOCR) and metabolites in platelets as measured by quantitative 1H-NMR metabolomics. Samples collected in ED at a single timepoint. WB and platelets isolated from the same blood samples. Comparison of mitochondrial function and metabolomics in patients with sepsis and non-sepsis ED patients |
| Institute | University of Michigan; University of Mississippi; University of Minnesota |
| Department | Clinical Pharmacy (UMich); Emergency Medicine (UMiss) |
| Laboratory | Stringer NMR Metabolomics Laboratory |
| Last Name | McHugh |
| First Name | Cora |
| Address | 428 Church St, Ann Arbor, MI, 48103, USA |
| NMRmetabolomics@umich.edu | |
| Phone | 7343530164 |
| Submit Date | 2019-12-13 |
| Num Groups | 2 |
| Total Subjects | 23 |
| Num Males | 12 |
| Num Females | 11 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2020-05-26 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO001363 |
| Collection Summary: | WB samples (12 mL each) were collected by direct venipuncture or from an indwelling line into K2 EDTA-containing tubes. Platelets were then isolated. centrifuged (200x gfor 6 min at room temperature).The platelet-rich plasma layer was transferred to another tube and centrifuged (4500x gfor 5 minat 4C) to pellet the platelets.The nearly cell-free plasma layer was transferred to a new tube, leaving approximately 0.25 mL of plasma in the original tube. Remaining plasma was used to resuspend the platelet pellet to produce ultra-rich plasma. |
| Sample Type: | Platelets |
| Collection Method: | direct venipuncture or indwelling line |
| Collection Frequency: | once |
| Volumeoramount Collected: | 12mL |
| Storage Conditions: | Described in summary |
| Collection Vials: | K2 EDTA-containing Vacutainer tubes (BD 134367863; Becton-Dickinson, Franklin Lakes, NJ USA) |
| Additives: | K2 EDTA |
