Summary of Study ST001807
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001141. The data can be accessed directly via it's Project DOI: 10.21228/M8711S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001807 |
| Study Title | Untargeted metabolomics of Daphnia magna exposed to a lithium cobalt oxide nanomaterial |
| Study Type | Untargeted MS |
| Study Summary | The goal of this project was to determine lithium cobalt oxide (LCO)’s effects on pathways in the model organism Daphnia magna through untargeted metabolomics. |
| Institute | University of Wisconsin - Milwaukee |
| Department | School of Freshwater Sciences |
| Laboratory | Rebecca Klaper |
| Last Name | Klaper |
| First Name | Rebecca |
| Address | 600 E Greenfield Ave, Milwaukee, WI 53204 |
| rklaper@uwm.edu | |
| Phone | 4143821713 |
| Submit Date | 2021-05-18 |
| Num Groups | 4 |
| Total Subjects | 36 |
| Study Comments | Pooled samples of 20 Daphnia magna per replicate. |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzXML |
| Analysis Type Detail | MS(Dir. Inf.) |
| Release Date | 2022-05-19 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Collection:
| Collection ID: | CO001877 |
| Collection Summary: | Daphnid cultures D. magna were cultured at a density of 20 animals per liter MHRW, 16 h:8 h light:dark photoperiod, and a temperature of 20 °C. For metabolomics, cultures were fed daily on suspensions of unicellular green alga, Chlorella vulgaris (7.84 × 107 cells/mL) at 2 mL/L. Algae was supplemented daily by 50 µL/L of dried bakers yeast (1mg/mL stock, Sigma–Aldrich). Cultures were maintained using third or fourth brood neonates less than 24 h old. Ten replicates were prepared for each treatment: MHRW control, 1 mg/L LCO, and ion control (66 μg/L Li as LiCl and 15 μg/L Co as CoCl2; equivalent to ions released by 1 mg/L LCO over 48 h).1,2 Twenty neonates per replicate (< 9 h old) were transferred to 100 ml beakers 48 hours prior to exposure and fed proportionate amounts of food for 48 hours. At the end of the 48-hour feeding period, daphnids were transferred to 200 ml control or exposure beakers. Exposures were carried out for 48 h without food per standard OECD guidelines for D. magna. At the end of the exposure daphnids were collected and transferred (20 pooled animals per replicate) into labeled Precellys tubes using a fine sable brush and flash frozen in liquid nitrogen.At the end of the exposure daphnids were collected and transferred (20 pooled animals per replicate) into labeled Precellys tubes using a fine sable brush and flash frozen in liquid nitrogen. Samples were stored at -80 °C and shipped to the University of Birmingham, UK on dry ice. |
| Sample Type: | Whole animals |
| Storage Conditions: | -80℃ |
| Collection Vials: | Precellys tubes |
