Summary of Study ST002055

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR001300. The data can be accessed directly via it's Project DOI: 10.21228/M8P69K This work is supported by NIH grant, U2C- DK119886.


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Study IDST002055
Study TitleMetabolomic Profiling of Human Pluripotent Stem Cell Differentiation into Lung Progenitors
Study SummaryMetabolism is vital to cellular function and tissue homeostasis during human lung development. In utero, embryonic pluripotent stem cells undergo endodermal differentiation towards a lung progenitor cell fate that can be mimicked in vitro using induced human pluripotent stem cells (hiPSCs) to study genetic mutations. To identify differences between wild type and surfactant protein B (SFTPB)-deficient cell lines during endoderm specification towards lung, we used an untargeted metabolomics approach to evaluate the developmental changes in metabolites. We found that the metabolites most enriched during the differentiation from pluripotent stem cell to lung progenitor cell, regardless of cell line, were sphingomyelins and phosphatidylcholines, two important lipid classes in fetal lung development. The SFTPB mutation had no metabolic impact on early endodermal lung development. The identified metabolite signatures during lung progenitor cell differentiation may be utilized as biomarkers for normal embryonic lung development.
The Hospital for Sick Children
Last NamePost
First NameMartin
Address555 University Avenue
Submit Date2021-06-29
Raw Data AvailableYes
Raw Data File Type(s)wiff
Analysis Type DetailLC-MS
Release Date2022-01-13
Release Version1
Martin Post Martin Post application/zip

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Collection ID:CO002130
Collection Summary:Samples were analyzed for 180 metabolites using the AbsoluteIDQ® p180 kit from Biocrates Life Sciences AG at the Analytical Facility for Bioactive Molecules (The Hospital for Sick Children, Toronto, Canada). Collected cell pellets were resuspended in 15 % ice-cold 10 mM Phosphate Buffer (PB) + 85 % EtOH as per AbsoluteIDQ® p180 kit protocol. Resuspended cells were subjected to three rounds of sonication and rapid freeze thawing. Final suspension was then centrifuged at 20,000 x g for 10 minutes at 4°C. A small aliquot was taken for protein assay, and the remainder of the supernatant was used for metabolic analysis. Samples, standards, and controls (10 mL each) were added to the Biocrates 96-well filter plate with internal standard, dried under nitrogen, derivatized with phenyl isothiocyanate, and then extracted with methanol, as per kit instructions.
Sample Type:human cells