Summary of Study ST002220
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001418. The data can be accessed directly via it's Project DOI: 10.21228/M8F409 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002220 |
Study Title | Catabolism of branched-chain amino acids (BCAAs) in renal cells HK2 and 786-O |
Study Summary | The objective of this experiment is to compare the catabolism of branched-chain amino acids (BCAAs) in human renal epithelial cell line HK2 versus ccRCC cell lines 786-O, 786-M1A and 786-M2A using 13C6-labelled leucine and isoleucine stable isotope tracers. To this end, we incubated the above cell lines with 13C6-leucine and 13C6-isoleucine in Plasmax media for 27h. Data were generated from 5 independent cultures. This is Part I of the study and the experimental number is MS42. |
Institute | CECAD Research Center |
Last Name | Yang |
First Name | Ming |
Address | Joseph-Stelzmann-Straße 26, CECAD Research Center, Köln, Koeln, 50931, Germany |
ming.yang@uni-koeln.de | |
Phone | +4922147884306 |
Submit Date | 2022-07-15 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2022-08-03 |
Release Version | 1 |
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Collection:
Collection ID: | CO002299 |
Collection Summary: | 2x105 cells were plated onto 6-well plates in Plasmax media (5 replicates for each cell type). The day after, fresh media containing 13C6-leucine and 13C6-isoleucine for 27h was applied. Before extraction, cells were counted using CASY cell counter (Omni Life Sciences) using a separate counting plate. After that, cells were washed at room temperature with PBS twice and then kept in a cold bath with dry ice and methanol before adding the metabolite extraction solution. |
Sample Type: | Cultured cells |