Summary of Study ST003399
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002105. The data can be accessed directly via it's Project DOI: 10.21228/M8HV52 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003399 |
| Study Title | Chemical Biology Meets Metabolomics: The Response of Barley Seedlings to 3,5-Dichloroanthranilic Acid, a Resistance Inducer |
| Study Type | Plant metabolomics |
| Study Summary | Advances in combinatorial synthesis and high-throughput screening methods have led to renewed interest in synthetic plant immune activators as well as priming agents. 3,5-Dichloroanthranilic acid (3,5-DCAA) is a derivative of anthranilic acid that have shown potency in activating defence mechanisms in Arabidopsis and barley plants. Chemical biology which is the interface of chemistry and biology can make use of metabolomics approaches and tools to better understand molecular mechanisms operating in complex biological systems. Aim: Here we report on the untargeted metabolomics profiling of barley seedlings treated with 3,5-DCAA to gain deeper insights into the mechanism of action of this resistance inducer. Methodology: Hydro-methanolic extracts from different time periods (12, 24 and 36 h post-treatment) were analysed on ultra-high performance liquid chromatography hyphenated with a high-resolution mass spectrometer. Both unsupervised and supervised chemometric methods were used to reveal hidden patterns and highlight metabolite variables associated with the treatment. Results: Based on the metabolites identified, both the phenylpropanoid and octadecanoid pathways appear to be main route activated by 3,5-DCAA. Different classes of responsive metabolites were annotated with favonoids, more especially flavones, the most dominant. Given the limited understanding of this inducer, this study offers a metabolomics analysis of the response triggered by its foliar application in barley. This additional insight could help make informed decision for the development of more effective strategies for crop protection and improvement, ultimately contributing to agricultural sustainability and resilience. |
| Institute | University of Johannesburg |
| Department | Biochemistry |
| Laboratory | Plant metabolomics |
| Last Name | Claude Yasmine Hamany Djande |
| First Name | Claude Yasmine |
| Address | 81A Fourth Avenue Westdene |
| claudehamany@gmail.com | |
| Phone | 0814415123 |
| Submit Date | 2024-07-05 |
| Num Groups | 4 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Waters) |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-09-03 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO003517 |
| Collection Summary: | Seeds from the barley cultivar ‘Elim’ were provided by the South African Barley Breeding Institute (SABBI, Bredasdorp, Western Cape, South Africa). They were surfaced-sterilised with 70% ethanol and soaked in sterile water for 2 h prior to cultivation in soil (Germination mix, Culterra, Muldersdrift, South Africa) pasteurised at 70 °C. An average of 40 seeds were planted in each pot (three pots per condition or three biological replicate) measuring 8 cm depth and 12 cm in diameter. The watering of the plants was performed twice a week with water and a solution containing a water-soluble chemical fertiliser (Multisol ‘N’, Culterra, Muldersdrift, South Africa). Seedlings were kept in a regulated growth environment with a 12-hour light-dark cycle at 22 to 27°C until 16 d post-emergence or 21 d after planting, corresponding to physiological stage 13 according to the Zadocks growth and development scale (Zadoks et al. 1974). The priming inducer 3,5-dichloroanthranilic acid (3,5-DCAA) was purchased from Merck-Sigma-Aldrich, (Johannesburg, South Africa). 3,5-DCAA was dissolved in dimethylsulphoxide (DMSO, 1 μL.mL-1; BDH Chemicals, UK) and mixed with 0.05% wetting agent (Effekto, Pretoria, South Africa) in distilled water to obtain the desired concentrations. Approximately 6 mL (40 sprays) of 200 μM 3,5-DCAA was applied on the leaf tissue of the seedlings while the controls received only the DMSO solution. The leaf material was harvested at 12, 24 and 36 h post-treatment and snap-frozen in liquid nitrogen to quench metabolic activity. Samples were stored in -80 °C for later use. Metabolites were extracted as previously described (Hamany Djande et al., 2023b). Briefly, the leaf tissue was ground with liquid nitrogen, then 80% methanol was added and the mixture was homogenized. Subsequently, all hydromethanolic samples were centrifuged, and the supernatant was concentrated and further evaporated to complete dryness. The dried extracts were dissolved in 50% methanol, filtered, and prepared for LC-MS analysis. |
| Sample Type: | Plant shoot tissue |
