Summary of Study ST003553
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002188. The data can be accessed directly via it's Project DOI: 10.21228/M8SV5J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003553 |
| Study Title | Investigation of age-dependent changes in the gut of honeybee workers using targeted metabolomics for amino acid and biogenic amine determination |
| Study Summary | In the honeybee guts we measured the concentrations of 20 amino acids and 6 biogenic amines. Tryptophan and its downstream metabolite kynurenine followed the same trends across the worker development, with a significant decrease in their concentrations from day 3 onwards. Serotonin, on the other hand, was significantly increased from day 1. Tyramine and octopamine showed significant increases in concentrations (octopamine for all age groups apart from Day 1; tyramine for all age groups), while their precursor tyrosine showed significantly decreased concentrations for all age groups except experienced foragers. |
| Institute | Helmholtz Centre for Environmental Research |
| Department | Molecular Systems Biology |
| Last Name | Engelmann |
| First Name | Beatrice |
| Address | Permoserstraße 15, Leipzipg, Saxony, 03418, Germany |
| beatrice.engelmann@ufz.de | |
| Phone | 004934160251099 |
| Submit Date | 2024-10-28 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-11-27 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO003675 |
| Collection Summary: | Workers were pooled to obtain a final sample number of n = 5. The brain(s) and gut(s) from the same workers were assigned the same sample names. In total, we had 15 samples from each age group, with the exception of the foraging stages, where owing to the lack of foragers from Hive 1, we only had 11. All sampled bees were placed directly in liquid nitrogen to prevent changes in the metabolic profile. They were then decapitated in the laboratory so that the head and body of each bee were kept in a separate Eppendorf tube (Eppendorf, Germany) for later dissection. Worker guts were dissected on Petri dishes. Sternite 6 and the stinger apparatus were gently pulled using tweezers, which allowed extraction of the entire gastrointestinal tract. The stinger apparatus and venom sac were carefully excised and the honey crop was removed. |
| Sample Type: | Bee gut |
