Summary of Study ST003554
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002188. The data can be accessed directly via it's Project DOI: 10.21228/M8SV5J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003554 |
| Study Title | Investigation of age-dependent changes in the brain of honeybee workers using targeted metabolomics for amino acid and biogenic amine determination |
| Study Summary | In the honeybee guts we measured the concentrations of 21 amino acids and 6 biogenic amines. Using a k-means clustering, the metabolites were grouped by their trends into six clusters. Tyramine and tyrosine are involved in the octopamine and dopamine biosynthesis. We found that all four metabolites were part of the same cluster, with their concentrations being significantly decreased in comparison to newly emerged workers from day 1 onwards for the entire duration of the study. |
| Institute | Helmholtz Centre for Environmental Research |
| Department | Molecular Systems Biology |
| Last Name | Engelmann |
| First Name | Beatrice |
| Address | Permoserstraße 15, Leipzipg, Saxony, 03418, Germany |
| beatrice.engelmann@ufz.de | |
| Phone | 004934160251099 |
| Submit Date | 2024-10-28 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-11-27 |
| Release Version | 1 |
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Collection:
| Collection ID: | CO003676 |
| Collection Summary: | Workers were pooled to obtain a final sample number of n = 5. The brain(s) and gut(s) from the same workers were assigned the same sample names. In total, we had 15 samples from each age group, with the exception of the foraging stages, where owing to the lack of foragers from Hive 1, we only had 11. All sampled bees were placed directly in liquid nitrogen to prevent changes in the metabolic profile. They were then decapitated in the laboratory so that the head and body of each bee were kept in a separate Eppendorf tube (Eppendorf, Germany) for later dissection. Honeybee brains were dissected using a dissecting microscope (SZX7, Olympus). The head was placed on a petri dish lined with SYLGARD® 184 silicone. Before pinning the head for dissection, antennae and mouthpieces were removed. Subsequently, double-distilled water (ddH2O) was added until the head was fully submerged to facilitate dissection. The head cuticle was removed, and the hypopharyngeal glands and trachea were extracted from the inside. The brain was then separated from the cuticle and remaining tracheal remnants were removed before transferring the clean brain to a new Eppendorf tube. |
| Sample Type: | Bee brain |
