Summary of Study ST000198
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000169. The data can be accessed directly via it's Project DOI: 10.21228/M8N88K This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000198 |
| Study Title | Liver and Plasma metaboites for 13C-glucose load in wild type, LIRKO and LIRFKO mice |
| Study Type | Acyl-carnitine analysis (plasma) |
| Study Summary | FoxO proteins are major targets of insulin action. To better define the role of FoxO1 in mediating insulin effects in the liver, we generated liver-specific insulin receptor knockout (LIRKO) and IR/FoxO1 double knockout (LIRFKO) mice. Here we show that LIRKO mice are severely insulin resistant based on glucose, insulin and C-peptide levels, and glucose and insulin tolerance tests, and genetic deletion of hepatic FoxO1 reverses these effects. 13C-glucose and insulin clamp studies indicate that regulation of both hepatic glucose production (HGP) and glucose utilization is impaired in LIRKO mice, and these defects are also restored in LIRFKO mice corresponding to changes in gene expression. We conclude that (1) inhibition of FoxO1 is critical for both direct (hepatic) and indirect effects of insulin on HGP and utilization, and (2) extrahepatic effects of insulin are sufficient to maintain normal whole-body and hepatic glucose metabolism when liver FoxO1 activity is disrupted. Research is published: http://www.nature.com/ncomms/2015/150512/ncomms8079/full/ncomms8079.html |
| Institute | University of Michigan |
| Department | Biomedical Research Core Facilities |
| Laboratory | Metabolomics core |
| Last Name | Kachman |
| First Name | Maureen |
| Address | 6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714 |
| mkachman@umich.edu | |
| Submit Date | 2015-06-10 |
| Num Groups | 5 |
| Total Subjects | 40 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | d |
| Uploaded File Size | 32 M |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-12-28 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Factors:
Subject type: Animal; Subject species: Mus musculus (Factor headings shown in green)
| mb_sample_id | local_sample_id | treatment | Strain |
|---|---|---|---|
| SA009991 | S00013165 | glucose | IRF floxed |
| SA009992 | S00013145 | glucose | IRF floxed |
| SA009985 | S00013144 | glucose | IR floxed |
| SA009986 | S00013164 | glucose | IR floxed |
| SA009987 | S00013163 | glucose | IR floxed |
| SA009988 | S00013162 | glucose | IR floxed |
| SA009989 | S00013142 | glucose | IR floxed |
| SA009990 | S00013143 | glucose | IR floxed |
| SA009993 | S00013154 | glucose | LIRFKO |
| SA009994 | S00013153 | glucose | LIRFKO |
| SA009995 | S00013175 | glucose | LIRFKO |
| SA009996 | S00013173 | glucose | LIRFKO |
| SA009997 | S00013152 | glucose | LIRFKO |
| SA009998 | S00013172 | glucose | LIRFKO |
| SA009999 | S00013174 | glucose | LIRFKO |
| SA010000 | S00013155 | glucose | LIRFKO |
| SA010001 | S00013166 | glucose | LIRKO |
| SA010002 | S00013146 | glucose | LIRKO |
| SA010003 | S00013151 | glucose | LIRKO |
| SA010004 | S00013167 | glucose | LIRKO |
| SA010005 | S00013168 | glucose | LIRKO |
| SA010006 | S00013171 | glucose | LIRKO |
| SA010007 | S00013170 | glucose | LIRKO |
| SA010008 | S00013169 | glucose | LIRKO |
| SA010009 | S00013148 | glucose | LIRKO |
| SA010010 | S00013147 | glucose | LIRKO |
| SA010011 | S00013150 | glucose | LIRKO |
| SA010012 | S00013149 | glucose | LIRKO |
| SA010013 | S00013140 | none | IR floxed |
| SA010014 | S00013139 | none | IR floxed |
| SA010015 | S00013137 | none | IR floxed |
| SA010016 | S00013138 | none | IR floxed |
| SA010017 | S00013136 | none | IR floxed |
| SA010018 | S00013156 | none | IR floxed |
| SA010019 | S00013159 | none | IR floxed |
| SA010020 | S00013160 | none | IR floxed |
| SA010021 | S00013141 | none | IR floxed |
| SA010022 | S00013158 | none | IR floxed |
| SA010023 | S00013157 | none | IR floxed |
| SA010024 | S00013161 | none | IR floxed |
| Showing results 1 to 40 of 40 |
