Summary of Study ST000302

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000154. The data can be accessed directly via it's Project DOI: 10.21228/M8688J This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

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Study IDST000302
Study TitleIsocitrate dehydrogenase-1/Glioma Fluxomics Study
Study SummaryTumor neurospheres were grown in culture until ~90% confluent. Media was changed to contain 1mM acetate. After 24h, 0h time points were collected and media was changed on all other cells to 1mM 13C-acetate containing media. Cells were then collected at their various time points, 1, 3, 24, 48, or 72 hours. Cells were collected into 15mL tubes, spun down at 100xg for 1min and media aspirated. Pellet was washed (not resuspended) in 150mM ammonium acetate. This was then aspirated off and the cells snap frozen and stored at -80C until all time points complete to ship on dry ice. 0h, 1h, and 3h A, B, and C samples will have gTn by HILIC + TCA by GCMS done on them, while 0h, 1h, and 3h D, E, and F will have FAMES and DG & PC analysis done on them. 24h, 48h, and 72h A, B, and C samples will have FAMES analysis done on them.
Institute
University of Michigan
DepartmentBiomedical Research Core Facilities
LaboratoryMetabolomics core
Last NameKachman
First NameMaureen
Address6300 Brehm Tower, 1000 Wall Street, Ann Arbor, MI 48105-5714
Emailmkachman@med.umich.edu
Phone(734) 232-8175
Submit Date2015-03-24
Num Groups6
Total Subjects54
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailGC/LC-MS
Release Date2016-06-18
Release Version1
Maureen Kachman Maureen Kachman
https://dx.doi.org/10.21228/M8688J
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Cell line Labelint time (hours)
SA013636S00017834pLKO 0
SA013637S00017833pLKO 0
SA013638S00017837pLKO 0
SA013639S00017838pLKO 0
SA013640S00017835pLKO 0
SA013641S00017836pLKO 0
SA013642S00017847pLKO 1
SA013643S00017845pLKO 1
SA013644S00017848pLKO 1
SA013645S00017846pLKO 1
SA013646S00017850pLKO 1
SA013647S00017849pLKO 1
SA013648S00017870pLKO 24
SA013649S00017871pLKO 24
SA013650S00017869pLKO 24
SA013651S00017858pLKO 3
SA013652S00017859pLKO 3
SA013653S00017862pLKO 3
SA013654S00017857pLKO 3
SA013655S00017861pLKO 3
SA013656S00017860pLKO 3
SA013657S00017876pLKO 48
SA013658S00017875pLKO 48
SA013659S00017877pLKO 48
SA013660S00017881pLKO 72
SA013661S00017882pLKO 72
SA013662S00017883pLKO 72
SA013663S00017841shIDH1 0
SA013664S00017839shIDH1 0
SA013665S00017842shIDH1 0
SA013666S00017840shIDH1 0
SA013667S00017843shIDH1 0
SA013668S00017844shIDH1 0
SA013669S00017853shIDH1 1
SA013670S00017854shIDH1 1
SA013671S00017856shIDH1 1
SA013672S00017852shIDH1 1
SA013673S00017851shIDH1 1
SA013674S00017855shIDH1 1
SA013675S00017872shIDH1 24
SA013676S00017874shIDH1 24
SA013677S00017873shIDH1 24
SA013678S00017864shIDH1 3
SA013679S00017868shIDH1 3
SA013680S00017865shIDH1 3
SA013681S00017866shIDH1 3
SA013682S00017863shIDH1 3
SA013683S00017867shIDH1 3
SA013684S00017879shIDH1 48
SA013685S00017880shIDH1 48
SA013686S00017878shIDH1 48
SA013687S00017886shIDH1 72
SA013688S00017885shIDH1 72
SA013689S00017884shIDH1 72
Showing results 1 to 54 of 54
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